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验证数据展示

  • WB analysis using 30986-1-AP

    WB analysis using 30986-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30986-1-AP (CLUH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of COS-7 using 30986-1-AP

    IF Staining of COS-7 using 30986-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed COS-7 cells using CLUH antibody (30986-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CLUH Polyclonal antibody

CLUH Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30986-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, monkey

应用

WB, IF/ICC, ELISA

别名

KIAA0664, Protein KIAA0664, FLJ23672, CLU1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, mouse kidney tissue
Positive IF/ICC detected inCOS-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30986-1-AP targets CLUH in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, monkey
免疫原 CLUH fusion protein Ag34275 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0664
别名 KIAA0664, Protein KIAA0664, FLJ23672, CLU1
观测分子量147-150 kDa
GenBank蛋白编号BC156940
基因名称 CLUH
Gene ID (NCBI) 23277
RRIDAB_3669806
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO75153
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clustered mitochondria homolog (CLUH) is a conserved RNA-binding protein, that binds transcripts encoding proteins involved in the respiratory chain, the tricarboxylic acid (TCA) cycle, and other mitochondrial metabolic pathways. Upon loss of CLUH, target mRNAs are subjected to faster decay, and their respective proteins are decreased in abundance. This leads to alterations in mitochondrial distribution (mitochondrial clustering as the gene name alludes to) mitochondrial cristae integrity, respiratory defects, loss of mtDNA, and decreased activity of TCA cycle enzymes. (PMID: 35559794)

实验方案

Product Specific Protocols
WB protocol for CLUH antibody 30986-1-APDownload protocol
IF protocol for CLUH antibody 30986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols