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CLYBL Polyclonal antibody

CLYBL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17314-1-AP

Print datasheet

Synonyms

citrate lyase beta like, CLB, CLYBL

验证数据展示

  • WB analysis using 17314-1-AP

    WB analysis using 17314-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17314-1-AP (CLYBL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17314-1-AP

    IHC staining of human liver using 17314-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17314-1-AP (CLYBL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLYBL antibody included
  • IHC staining of human liver using 17314-1-AP

    IHC staining of human liver using 17314-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17314-1-AP (CLYBL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLYBL antibody included

经过测试的应用

Positive WB detected inHepG2 cells, human heart tissue, Jurkat cells, THP-1 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CLYBL Ready-To-Use IHC Kit. CLYBL primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17314-1-AP targets CLYBL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen CLYBL fusion protein Ag11283 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name citrate lyase beta like
Synonyms citrate lyase beta like, CLB, CLYBL
Calculated Molecular Weight 340 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC034360
Gene Symbol CLYBL
Gene ID (NCBI) 171425
RRIDAB_2083483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N0X4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLYBL, also named as CLB, is a Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism (PubMed:29056341). It is a human mitochondrial enzyme of unknown function that is found in multiple eukaryotic taxa and conserved to bacteria. It acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. There're two isoforms with MW 31-33 kDa and 35-37 kDa.

实验方案

Product Specific Protocols
WB protocol for CLYBL antibody 17314-1-APDownload protocol
IHC protocol for CLYBL antibody 17314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Eur J Immunol

YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.

Authors - Satabdi Nandi
View Article