验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, human heart tissue, human placenta tissue, MCF-7 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18189-1-AP targets Mast Cell Chymase in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Mast Cell Chymase fusion protein Ag12909 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chymase 1, mast cell |
| 别名 | Alpha chymase, chymase, chymase 1, mast cell, CMA1, CYH, CYM, Mast Cell Chymase, Mast cell protease I, Mcpt 5, Mcpt5, MCT1 |
| 计算分子量 | 247 aa, 27 kDa |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | BC069370 |
| 基因名称 | Mast Cell Chymase |
| Gene ID (NCBI) | 1215 |
| RRID | AB_2083611 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23946 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mast Cell Chymase, also named as CYH, CYM and CMA1, belongs to the peptidase S1 family and Granzyme subfamily. It is the major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. CMA1 maybe a target for cardiovascular disease therapies.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Mast Cell Chymase antibody 18189-1-AP | Download protocol |
| IHC protocol for Mast Cell Chymase antibody 18189-1-AP | Download protocol |
| IP protocol for Mast Cell Chymase antibody 18189-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Perinatal androgens organize sex differences in mast cells and attenuate anaphylaxis severity into adulthood. | ||
Arterioscler Thromb Vasc Biol Stabilization of Perivascular Mast Cells by Endothelial CNP (C-Type Natriuretic Peptide). | ||
Int J Mol Sci Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa. | ||
Cell Cycle Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device. | ||
Sci Rep Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth. | ||
