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CMAS Polyclonal antibody

CMAS Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13006-1-AP

Print datasheet

Synonyms

CMAS, CMP Neu5Ac SYNTHETASE, CMP NeuNAc synthase

验证数据展示

  • WB analysis using 13006-1-AP

    WB analysis using 13006-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 13006-1-AP

    WB analysis using 13006-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13006-1-AP (CMAS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat pancreas tissue, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13006-1-AP targets CMAS in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CMAS fusion protein Ag3686 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytidine monophosphate N-acetylneuraminic acid synthetase
Synonyms CMAS, CMP Neu5Ac SYNTHETASE, CMP NeuNAc synthase
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC016609
Gene Symbol CMAS
Gene ID (NCBI) 55907
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytidine monophosphate N-acetylneuraminic acid synthetase (CMAS), a key enzyme in the incorporation pathway, catalyzes the synthesis of CMP-sialic acid by utilizing CTP and sialic acid. Neu5Ac is activated in the nucleus by CMAS to form CMP-Neu5Ac, which is transported to the Golgi apparatus by SLC35A1 for sialylation of glycoproteins and gangliosides catalyzed by specific sialy ltransferases (PMID: 30568043). Low CMAS gene expression correlated with reduced recurrence-free survival in a human colorectal cancer cohort (PMID: 30578646). Western blots indicated a range of bands at ~ 48 kDa.

实验方案

Product Specific Protocols
WB protocol for CMAS antibody 13006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols