UMP/CMP kinase Polyclonal antibody
UMP/CMP kinase Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, human pancreas tissue, human skeletal muscle tissue |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CMPK1 Ready-To-Use IHC Kit. CMPK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
11360-1-AP targets UMP/CMP kinase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | UMP/CMP kinase fusion protein Ag1920 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cytidine monophosphate (UMP-CMP) kinase 1, cytosolic |
| Synonyms | CMPK1, Cytidylate kinase, Cytidine monophosphate kinase, CMPK, CMK |
| Calculated Molecular Weight | 228 aa, 26 kDa |
| Observed Molecular Weight | 22-26 kDa |
| GenBank Accession Number | BC014961 |
| Gene Symbol | CMPK1 |
| Gene ID (NCBI) | 51727 |
| RRID | AB_2081395 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30085 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UMP/CMP kinase antibody 11360-1-AP | Download protocol |
| IHC protocol for UMP/CMP kinase antibody 11360-1-AP | Download protocol |
| IF protocol for UMP/CMP kinase antibody 11360-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Proteomics Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry. | ||
J Exp Clin Cancer Res Pericytes recruited by CCL28 promote vascular normalization after anti-angiogenesis therapy through RA/RXRA/ANGPT1 pathway in lung adenocarcinoma |
