验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
25877-1-AP targets CMPK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CMPK2 fusion protein Ag22885 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytidine monophosphate (UMP-CMP) kinase 2, mitochondrial |
| 别名 | UMP-CMP kinase 2, mitochondrial, UMP CMPK2, TYKi, Nucleoside-diphosphate kinase, EC:2.7.4.6 |
| 计算分子量 | 449 aa, 49 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC141802 |
| 基因名称 | CMPK2 |
| Gene ID (NCBI) | 129607 |
| RRID | AB_3085825 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5EBM0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CMPK2 belongs to the thymidylate kinase family. CMPK2 may participate in dUTP and dCTP synthesis in mitochondria. It is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Efficacy of CMPK2 is highest for dUMP followed by dCMP; CMP and UMP are poor substrates. CMPK2 may be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CMPK2 antibody 25877-1-AP | Download protocol |
| IHC protocol for CMPK2 antibody 25877-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Genet Mitochondrial-related hub genes in dermatomyositis: muscle and skin datasets-based identification and in vivo validation | ||
Sci Rep LincRNA-p21/AIF-1/CMPK2/NLRP3 pathway promoted inflammation, autophagy and apoptosis of human tubular epithelial cell induced by urate via exosomes | ||
Cell Biochem Biophys CMPK2 Promotes CD4+ T Cell Activation and Apoptosis through Modulation of Mitochondrial Dysfunction in Systemic Lupus Erythematosus
| ||
Int J Biol Sci The FTO-CMPK2 Pathway in Fibroblast-like Synoviocytes Modulates Rheumatoid Arthritis Synovial Inflammation and Cartilage Homeostasis via mtDNA Regulation |