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验证数据展示

  • WB analysis using 12103-1-AP

    WB analysis using 12103-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12103-1-AP (CNBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 12103-1-AP

    IF Staining of HeLa using 12103-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CNBP antibody (12103-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

CNBP Polyclonal antibody

CNBP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12103-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

RNF163, PROMM, DM2, CNBP1, Cellular nucleic acid-binding protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12103-1-AP targets CNBP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CNBP fusion protein Ag2747 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CCHC-type zinc finger, nucleic acid binding protein
Synonyms RNF163, PROMM, DM2, CNBP1, Cellular nucleic acid-binding protein
Calculated Molecular Weight 170 aa, 19 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC014911
Gene Symbol CNBP
Gene ID (NCBI) 7555
RRIDAB_3085390
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62633
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cellular nucleic acid binding protein (CNBP) has been implicated in vertebrate craniofacial development and in myotonic dystrophy type 2 (DM2) and sporadic inclusion body myositis (sIBM) human diseases [PMID:21277287]. CNBP is a small and strikingly conserved single-stranded nucleic acid binding protein that is able to bind DNA as well as RNA. This ssDNA- and RNA-binding protein with broad sequence specificity is participated in diverse cellular functions, including transcription, translation and through them cell proliferation and survival [PMID:20960530].

实验方案

Product Specific Protocols
WB protocol for CNBP antibody 12103-1-APDownload protocol
IF protocol for CNBP antibody 12103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols