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CNBP Polyclonal antibody

CNBP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12103-1-AP

Print datasheet

Synonyms

RNF163, PROMM, DM2, CNBP1, Cellular nucleic acid-binding protein

验证数据展示

  • WB analysis using 12103-1-AP

    WB analysis using 12103-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12103-1-AP (CNBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 12103-1-AP

    IF Staining of HeLa using 12103-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CNBP antibody (12103-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12103-1-AP targets CNBP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CNBP fusion protein Ag2747 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CCHC-type zinc finger, nucleic acid binding protein
Synonyms RNF163, PROMM, DM2, CNBP1, Cellular nucleic acid-binding protein
Calculated Molecular Weight 170 aa, 19 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC014911
Gene Symbol CNBP
Gene ID (NCBI) 7555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62633
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cellular nucleic acid binding protein (CNBP) has been implicated in vertebrate craniofacial development and in myotonic dystrophy type 2 (DM2) and sporadic inclusion body myositis (sIBM) human diseases [PMID:21277287]. CNBP is a small and strikingly conserved single-stranded nucleic acid binding protein that is able to bind DNA as well as RNA. This ssDNA- and RNA-binding protein with broad sequence specificity is participated in diverse cellular functions, including transcription, translation and through them cell proliferation and survival [PMID:20960530].

实验方案

Product Specific Protocols
WB protocol for CNBP antibody 12103-1-APDownload protocol
IF protocol for CNBP antibody 12103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols