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CNDP1 Polyclonal antibody

CNDP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17759-1-AP

Print datasheet

Synonyms

CPGL2, CNDP dipeptidase 1, CN1, Carnosine dipeptidase 1, Beta-Ala-His dipeptidase

验证数据展示

  • WB analysis of HEK-293 using 17759-1-AP

    WB analysis of HEK-293 using 17759-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17759-1-AP (CNDP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 17759-1-AP

    IHC staining of human prostate cancer using 17759-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 17759-1-AP

    IHC staining of human prostate cancer using 17759-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 17759-1-AP

    IF Staining of A431 using 17759-1-AP

    Immunofluorescent analysis of A431 cells using 17759-1-AP (CNDP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells
Positive IHC detected inhuman prostate cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17759-1-AP targets CNDP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CNDP1 fusion protein Ag12127 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carnosine dipeptidase 1 (metallopeptidase M20 family)
Synonyms CPGL2, CNDP dipeptidase 1, CN1, Carnosine dipeptidase 1, Beta-Ala-His dipeptidase
Calculated Molecular Weight 508 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC110295
Gene Symbol CNDP1
Gene ID (NCBI) 84735
RRIDAB_2878435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KN2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-Ala-His dipeptidase (CNDP1) encodes the secreted serum carnosinase, which is synthesized in the brain and secreted into the systematic circulation, CNDP1 hydrolyzes L-carnosine and is essential for glucose metabolism and the transportation of activated fatty acids across the mitochondrial membrane (PMID: 32111749, 16046297).

实验方案

Product Specific Protocols
WB protocol for CNDP1 antibody 17759-1-APDownload protocol
IHC protocol for CNDP1 antibody 17759-1-APDownload protocol
IF protocol for CNDP1 antibody 17759-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Amino Acids

Serum and urinary carnosinase-1 correlate with kidney function and inflammation

Authors - Shiqi Zhang
View Article