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验证数据展示

  • WB analysis of HEK-293 using 17759-1-AP

    WB analysis of HEK-293 using 17759-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17759-1-AP (CNDP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 17759-1-AP

    IHC staining of human prostate cancer using 17759-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 17759-1-AP

    IHC staining of human prostate cancer using 17759-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17759-1-AP (CNDP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 17759-1-AP

    IF Staining of A431 using 17759-1-AP

    Immunofluorescent analysis of A431 cells using 17759-1-AP (CNDP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CNDP1 Polyclonal antibody

CNDP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17759-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CPGL2, CNDP dipeptidase 1, CN1, Carnosine dipeptidase 1, Beta-Ala-His dipeptidase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells
Positive IHC detected inhuman prostate cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17759-1-AP targets CNDP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CNDP1 fusion protein Ag12127 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carnosine dipeptidase 1 (metallopeptidase M20 family)
别名 CPGL2, CNDP dipeptidase 1, CN1, Carnosine dipeptidase 1, Beta-Ala-His dipeptidase
计算分子量 508 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC110295
基因名称 CNDP1
Gene ID (NCBI) 84735
RRIDAB_2878435
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96KN2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-Ala-His dipeptidase (CNDP1) encodes the secreted serum carnosinase, which is synthesized in the brain and secreted into the systematic circulation, CNDP1 hydrolyzes L-carnosine and is essential for glucose metabolism and the transportation of activated fatty acids across the mitochondrial membrane (PMID: 32111749, 16046297).

实验方案

Product Specific Protocols
WB protocol for CNDP1 antibody 17759-1-APDownload protocol
IHC protocol for CNDP1 antibody 17759-1-APDownload protocol
IF protocol for CNDP1 antibody 17759-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Amino Acids

Serum and urinary carnosinase-1 correlate with kidney function and inflammation

Authors - Shiqi Zhang
View Article