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  • Featured Product
  • KD/KO Validated

CNN3 Polyclonal antibody

CNN3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11509-1-AP

Print datasheet

Synonyms

CNN 3, Calponin-3, Calponin3, Calponin 3

验证数据展示

  • WB analysis of HEK-293 using 11509-1-AP

    WB analysis of HEK-293 using 11509-1-AP

    WB result of CNN3 antibody (11509-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN3 transfected HEK-293 cells.

  • WB analysis using 11509-1-AP

    WB analysis using 11509-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of human kidney using 11509-1-AP

    WB analysis of human kidney using 11509-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11509-1-AP

    IHC staining of human prostate cancer using 11509-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CNN3 antibody included
  • IHC staining of human prostate cancer using 11509-1-AP

    IHC staining of human prostate cancer using 11509-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CNN3 antibody included
  • IP experiment of HEK-293 using 11509-1-AP

    IP experiment of HEK-293 using 11509-1-AP

    IP result of anti-CNN3 (IP:11509-1-AP, 4ug; Detection:11509-1-AP 1:15000) with HEK-293 cells lysate 960 ug.

  • IF Staining of HeLa using 11509-1-AP

    IF Staining of HeLa using 11509-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNN3 antibody (11509-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IHC staining of human stomach cancer using 11509-1-AP

    IHC staining of human stomach cancer using 11509-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CNN3 antibody included

经过测试的应用

Positive WB detected inHCT 116 cells, HAP1 cells, human kidney tissue, human skeletal muscle tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells, U-87MG cells
Positive IHC detected inhuman prostate cancer tissue, human hysteromyoma tissue, human skin tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CNN3 Ready-To-Use IHC Kit. CNN3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11509-1-AP targets CNN3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CNN3 fusion protein Ag2068 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calponin 3, acidic
Synonyms CNN 3, Calponin-3, Calponin3, Calponin 3
Calculated Molecular Weight 329 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC025372
Gene Symbol CNN3
Gene ID (NCBI) 1266
RRIDAB_2082140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15417
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CNN3 antibody 11509-1-APDownload protocol
IHC protocol for CNN3 antibody 11509-1-APDownload protocol
IF protocol for CNN3 antibody 11509-1-APDownload protocol
IP protocol for CNN3 antibody 11509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Rep

Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.

Authors - Taha A Jan
View Article
humanWB

J Proteome Res

Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma

Authors - Pei-Shan Wu
View Article
mouseIHC

Mol Cell Biochem

STAT3 mediates ECM stiffness-dependent progression in ovarian cancer

Authors - Sun Chenchen
View Article
humanWB

Gastroenterol Res Pract

PMID: 30911294

Authors - Keun-Seok Hong
  • KD Validated
View Article