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CNNM3 Polyclonal antibody

CNNM3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13976-1-AP

Print datasheet

Synonyms

Cyclin-M3, cyclin M3, Ancient conserved domain-containing protein 3, ACDP3

验证数据展示

  • IHC staining of human spleen using 13976-1-AP

    IHC staining of human spleen using 13976-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 13976-1-AP (CNNM3 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of K-562 using 13976-1-AP

    IP experiment of K-562 using 13976-1-AP

    IP result of anti-CNNM3 (IP:13976-1-AP, 4ug; Detection:13976-1-AP 1:500) with K-562 cells lysate 3600ug.

  • WB analysis using 13976-1-AP

    WB analysis using 13976-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13976-1-AP (CNNM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 13976-1-AP

    IF Staining of A431 using 13976-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CNNM3 antibody (13976-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, K-562 cells, Raji cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13976-1-AP targets CNNM3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CNNM3 fusion protein Ag5012 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin M3
Synonyms Cyclin-M3, cyclin M3, Ancient conserved domain-containing protein 3, ACDP3
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC037272
Gene Symbol CNNM3
Gene ID (NCBI) 26505
RRIDAB_2082153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NE01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human Cyclin M 3 (CNNM3) has five transmembrane domains located at the N-terminal region and a C terminus containing a CBS pair domain and a cyclin box, both of which are part of the ACD region. CNNM3 magnesium transporter raises the intracellular magnesium content of cancer cells contributing to increased tumor growth and the acquisition of oncogenic properties (PMID: 24632616).

实验方案

Product Specific Protocols
WB protocol for CNNM3 antibody 13976-1-APDownload protocol
IHC protocol for CNNM3 antibody 13976-1-APDownload protocol
IF protocol for CNNM3 antibody 13976-1-APDownload protocol
IP protocol for CNNM3 antibody 13976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Oncogene

The protein tyrosine phosphatase PRL-2 interacts with the magnesium transporter CNNM3 to promote oncogenesis.

Authors - S Hardy
View Article
mouseWB,IF

JCI Insight

PRL2 links magnesium flux and sex-dependent circadian metabolic rhythms.

Authors - Noriko Uetani
View Article
humanWB,IP

Proc Natl Acad Sci U S A

PRL-1/2 phosphatases control TRPM7 magnesium-dependent function to regulate cellular bioenergetics

Authors - Serge Hardy
View Article