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验证数据展示

  • IHC staining of human spleen using 13976-1-AP

    IHC staining of human spleen using 13976-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 13976-1-AP (CNNM3 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with CNNM3 antibody included
  • IP experiment of K-562 using 13976-1-AP

    IP experiment of K-562 using 13976-1-AP

    IP result of anti-CNNM3 (IP:13976-1-AP, 4ug; Detection:13976-1-AP 1:500) with K-562 cells lysate 3600ug.

  • WB analysis using 13976-1-AP

    WB analysis using 13976-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13976-1-AP (CNNM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 13976-1-AP

    IF Staining of A431 using 13976-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CNNM3 antibody (13976-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CNNM3 Polyclonal antibody

CNNM3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13976-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

Cyclin-M3, cyclin M3, Ancient conserved domain-containing protein 3, ACDP3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, K-562 cells, Raji cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CNNM3 Ready-To-Use IHC Kit. CNNM3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13976-1-AP targets CNNM3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IP, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 CNNM3 fusion protein Ag5012 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin M3
别名 Cyclin-M3, cyclin M3, Ancient conserved domain-containing protein 3, ACDP3
计算分子量 76 kDa
观测分子量 85 kDa
GenBank蛋白编号BC037272
基因名称 CNNM3
Gene ID (NCBI) 26505
RRIDAB_2082153
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NE01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human Cyclin M 3 (CNNM3) has five transmembrane domains located at the N-terminal region and a C terminus containing a CBS pair domain and a cyclin box, both of which are part of the ACD region. CNNM3 magnesium transporter raises the intracellular magnesium content of cancer cells contributing to increased tumor growth and the acquisition of oncogenic properties (PMID: 24632616).

实验方案

Product Specific Protocols
WB protocol for CNNM3 antibody 13976-1-APDownload protocol
IHC protocol for CNNM3 antibody 13976-1-APDownload protocol
IF protocol for CNNM3 antibody 13976-1-APDownload protocol
IP protocol for CNNM3 antibody 13976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Oncogene

The protein tyrosine phosphatase PRL-2 interacts with the magnesium transporter CNNM3 to promote oncogenesis.

Authors - S Hardy
View Article
mouseWB,IF

JCI Insight

PRL2 links magnesium flux and sex-dependent circadian metabolic rhythms.

Authors - Noriko Uetani
View Article
humanWB,IP

Proc Natl Acad Sci U S A

PRL-1/2 phosphatases control TRPM7 magnesium-dependent function to regulate cellular bioenergetics

Authors - Serge Hardy
View Article