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CNNM4 Polyclonal antibody

CNNM4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14066-1-AP

Print datasheet

Synonyms

KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4

验证数据展示

  • WB analysis using 14066-1-AP

    WB analysis using 14066-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14066-1-AP (CNNM4 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 14066-1-AP

    IP experiment of mouse testis using 14066-1-AP

    IP result of anti-CNNM4 (IP:14066-1-AP, 4ug; Detection:14066-1-AP 1:1000) with mouse testis tissue lysate 4400ug.

  • IHC staining of human colon using 14066-1-AP

    IHC staining of human colon using 14066-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14066-1-AP (CNNM4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 14066-1-AP

    IF Staining of MCF-7 using 14066-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CNNM4 antibody (14066-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse colon tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14066-1-AP targets CNNM4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen CNNM4 fusion protein Ag5121 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin M4
Synonyms KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC063295
Gene Symbol CNNM4
Gene ID (NCBI) 26504
RRIDAB_2878009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P4Q7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).

实验方案

Product Specific Protocols
WB protocol for CNNM4 antibody 14066-1-APDownload protocol
IHC protocol for CNNM4 antibody 14066-1-APDownload protocol
IF protocol for CNNM4 antibody 14066-1-APDownload protocol
IP protocol for CNNM4 antibody 14066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Nat Commun

Restoring cellular magnesium balance through Cyclin M4 protects against acetaminophen-induced liver damage

Authors - Irene González-Recio
View Article