验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse colon tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
14066-1-AP targets CNNM4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | CNNM4 fusion protein Ag5121 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin M4 |
| 别名 | KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4 |
| 计算分子量 | 87 kDa |
| 观测分子量 | 90-100 kDa |
| GenBank蛋白编号 | BC063295 |
| 基因名称 | CNNM4 |
| Gene ID (NCBI) | 26504 |
| RRID | AB_2878009 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6P4Q7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNNM4 antibody 14066-1-AP | Download protocol |
| IHC protocol for CNNM4 antibody 14066-1-AP | Download protocol |
| IF protocol for CNNM4 antibody 14066-1-AP | Download protocol |
| IP protocol for CNNM4 antibody 14066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |