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CNNM4 Recombinant antibody

CNNM4 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240873E9

Cat no : 83824-4-RR

Print datasheet

Synonyms

KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4

验证数据展示

  • WB analysis using 83824-4-RR

    WB analysis using 83824-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83824-4-RR (CNNM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83824-4-RR

    Affinity and Kinetic Characterization of 83824-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83824-4-RR against Human CNNM4 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83824-4-RR is CNNM4 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CNNM4 fusion protein Ag5121 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cyclin M4
Synonyms KIAA1592, Cyclin-M4, cyclin M4, Ancient conserved domain-containing protein 4, ACDP4
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC063295
Gene Symbol CNNM4
Gene ID (NCBI) 26504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6P4Q7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ancient conserved domain-containing protein 4/cyclin M4 (CNNM4), a membrane protein that stimulates Mg2+ efflux, binds phosphatase of regenerating liver (PRL), which is frequently overexpressed in malignant human cancers (PMID: 25347473). CNNM4 is predominant in the brain, bone marrow, immune system, and especially abundant in the intestinal tract. CNNM4 localizes at the basolateral membrane of the intestinal epithelia and mediates transcellular Mg2+ transport (PMID: 27006114).

实验方案

Product Specific Protocols
WB protocol for CNNM4 antibody 83824-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols