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CNOT1 Polyclonal antibody

CNOT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14276-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

hNOT1, CDC39, CCR4-NOT transcription complex subunit 1, CCR4-associated factor 1, AD-005

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman brain tissue, HEK293 cells, mouse kidney tissue, mouse spleen tissue, mouse thymus tissue, HEK-293 cells, mouse brain tissue, mouse heart tissue, HeLa cells
Positive IP detected inmouse kidney tissue, HeLa cells
Positive IHC detected inhuman heart tissue, human spleen tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT1 Ready-To-Use IHC Kit. CNOT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14276-1-AP targets CNOT1 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, chIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, pig
免疫原 CNOT1 fusion protein Ag5623 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CCR4-NOT transcription complex, subunit 1
别名 hNOT1, CDC39, CCR4-NOT transcription complex subunit 1, CCR4-associated factor 1, AD-005
计算分子量 267 kDa
观测分子量 240-250 kDa, 174 kDa
GenBank蛋白编号BC040523
基因名称 CNOT1
Gene ID (NCBI) 23019
RRIDAB_10888627
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA5YKK6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms.This is a rabbit polyclonal antibody raised against part chain of C-terminal CNOT1 of human origin. CNOT1 exsits as many isoforms and molecular weight of isoforms are 267, 241 and 173 kDa.

实验方案

Product Specific Protocols
WB protocol for CNOT1 antibody 14276-1-APDownload protocol
IHC protocol for CNOT1 antibody 14276-1-APDownload protocol
IF protocol for CNOT1 antibody 14276-1-APDownload protocol
IP protocol for CNOT1 antibody 14276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.

Authors - Masashi Yamaji
humanWB

Cell

Roquin promotes constitutive mRNA decay via a conserved class of stem-loop recognition motifs.

Authors - Leppek Kathrin K
mouseWB

Cell

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Authors - Deniz Simsek
humanWB

Nat Cancer

SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers

Authors - Lei Shi
WB

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
humanWB

Mol Cell

Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation

Authors - Markus Höpfler
  • KD Validated
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