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验证数据展示

  • WB analysis of HeLa using 66507-1-Ig (same clone as 66507-1-PBS)

    WB analysis of HeLa using 66507-1-Ig (same clone as 66507-1-PBS)

    WB result of CNOT1 antibody (66507-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT1 transfected HeLa cells. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66507-1-Ig (same clone as 66507-1-PBS)

    IHC staining of human heart using 66507-1-Ig (same clone as 66507-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66507-1-Ig (CNOT1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CNOT1 antibody included
  • IF Staining of HepG2 using 66507-1-Ig (same clone as 66507-1-PBS)

    IF Staining of HepG2 using 66507-1-Ig (same clone as 66507-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66507-1-Ig (CNOT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

    WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

    WB analysis using 66507-1-Ig (same clone as 66507-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66507-1-Ig (CNOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66507-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CNOT1 Monoclonal antibody, PBS Only

CNOT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66507-1-PBS

产品说明书

CloneNo. 1A10A11

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

AD 005, CCR4 associated factor 1, CDC39, CNOT1, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, hNOT1, KIAA1007, NOT1, NOT1H

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CNOT1 Ready-To-Use IHC Kit. CNOT1 primary antibody included.

产品信息

66507-1-PBS targets CNOT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 CNOT1 fusion protein Ag5623 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CCR4-NOT transcription complex, subunit 1
别名 AD 005, CCR4 associated factor 1, CDC39, CNOT1, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, hNOT1, KIAA1007, NOT1, NOT1H
计算分子量 267 kDa
观测分子量 240-250 kDa
GenBank蛋白编号BC040523
基因名称 CNOT1
Gene ID (NCBI) 23019
RRIDAB_2881870
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDA5YKK6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.