验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy CNOT2 Ready-To-Use IHC Kit. CNOT2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10313-1-AP targets CNOT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | CNOT2 fusion protein Ag0347 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CCR4-NOT transcription complex, subunit 2 |
| 别名 | HSPC131, FLJ26456, CDC36, CCR4-NOT transcription complex subunit 2, CCR4-associated factor 2 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC002597 |
| 基因名称 | CNOT2 |
| Gene ID (NCBI) | 4848 |
| RRID | AB_2245072 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZN8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Ccr4-Not complex is evolutionarily conserved and controls gene expression both at the level of transcription initiation and of mRNA degradation. And this complex can regulate pol II transcription. Promoter targeting of CCR4-NOT transcription complex subunit 2 (CNOT2) results in strong repression of pol II transcription. CNOT2-mediated repression is sensitive to the HDAC (histone deacetylase) inhibitor, TSA (trichostatin A).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT2 antibody 10313-1-AP | Download protocol |
| IF protocol for CNOT2 antibody 10313-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cells Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation.
| ||
RNA Biol Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability. | ||
Stem Cells Dev Identification of Ccr4-Not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.
| ||
bioRxiv Cnot3 is required for male germ cell development and spermatogonial stem cell maintenance | ||
Methods Mol Biol Dissecting the Role of the Ccr4-Not Deadenylase Complex in Pluripotency and Differentiation |