验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, HEK-293 cells, HT-1080 cells, human brain tissue, K-562 cells, mouse brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11135-1-AP targets CNOT3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CNOT3 fusion protein Ag1587 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CCR4-NOT transcription complex, subunit 3 |
| 别名 | NOT3, LENG2, KIAA0691, CCR4-NOT transcription complex subunit 3, CCR4-associated factor 3 |
| 计算分子量 | 120 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC016474 |
| 基因名称 | CNOT3 |
| Gene ID (NCBI) | 4849 |
| RRID | AB_2229682 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75175 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and leads to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell apoptosis, proliferation, and metabolism [PMID:22342980]. CNOT3, belongs to the CCR4-NOT complex, has a critical role in maintaining mouse and human ESC identity as a protein complex and inhibit differentiation into the extraembryonic lineages. It is also involved in the regulation of the spindle assembly checkpoint [PMID:22342980,22367759].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT3 antibody 11135-1-AP | Download protocol |
| IF protocol for CNOT3 antibody 11135-1-AP | Download protocol |
| IP protocol for CNOT3 antibody 11135-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell A global in vivo Drosophila RNAi screen identifies NOT3 as a conserved regulator of heart function.
| ||
Nat Cancer ENO1 suppresses cancer cell ferroptosis by degrading the mRNA of iron regulatory protein 1. | ||
bioRxiv Cnot3 is required for male germ cell development and spermatogonial stem cell maintenance |
