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CNOT4 Polyclonal antibody

CNOT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 12564-1-AP

Print datasheet

Synonyms

EC:2.3.2.27, E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4

验证数据展示

  • WB analysis of mouse skeletal muscle using 12564-1-AP

    WB analysis of mouse skeletal muscle using 12564-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12564-1-AP (CNOT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 12564-1-AP

    WB analysis of MCF-7 using 12564-1-AP

    WB result of CNOT4 antibody (12564-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT4 transfected MCF-7 cells.

  • IHC staining of human lung cancer using 12564-1-AP

    IHC staining of human lung cancer using 12564-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12564-1-AP (CNOT4 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CNOT4 antibody included
  • IF Staining of HepG2 using 12564-1-AP

    IF Staining of HepG2 using 12564-1-AP

    Immunofluorescent analysis of HepG2 cells, using CNOT4 antibody 12564-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT4 Ready-To-Use IHC Kit. CNOT4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12564-1-AP targets CNOT4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CNOT4 fusion protein Ag3163 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CCR4-NOT transcription complex, subunit 4
Synonyms EC:2.3.2.27, E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4
Calculated Molecular Weight 575 aa, 64 kDa
Observed Molecular Weight 64-85 kDa
GenBank Accession NumberBC035590
Gene Symbol CNOT4
Gene ID (NCBI) 4850
RRIDAB_2082457
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95628
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CNOT4 antibody 12564-1-APDownload protocol
IHC protocol for CNOT4 antibody 12564-1-APDownload protocol
IF protocol for CNOT4 antibody 12564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
View Article
humanWB

J Cell Sci

Coupled protein quality control during nonsense-mediated mRNA decay

Authors - Alison J Inglis
View Article
humanWB,IF

Cells

Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability

Authors - Nafiseh Chalabi Hagkarim
  • KD Validated
View Article