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  • Featured Product
  • KD/KO Validated

CNOT9 Polyclonal antibody, PBS Only

CNOT9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22503-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HepG2 using 22503-1-AP (same clone as 22503-1-PBS)

    WB analysis of HepG2 using 22503-1-AP (same clone as 22503-1-PBS)

    WB result of RQCD1 antibody (22503-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RQCD1 transfected HepG2 cells. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • WB analysis using 22503-1-AP (same clone as 22503-1-PBS)

    WB analysis using 22503-1-AP (same clone as 22503-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22503-1-AP (CNOT9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse thymus using 22503-1-AP (same clone as 22503-1-PBS)

    WB analysis of mouse thymus using 22503-1-AP (same clone as 22503-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22503-1-AP (RQCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 22503-1-AP (same clone as 22503-1-PBS)

    IP experiment of MCF-7 using 22503-1-AP (same clone as 22503-1-PBS)

    IP result of anti-CNOT9 (IP:22503-1-AP, 4ug; Detection:22503-1-AP 1:1000) with MCF-7 cells lysate 1280ug. This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 22503-1-AP (same clone as 22503-1-PBS)

    IF Staining of HepG2 using 22503-1-AP (same clone as 22503-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CNOT9 antibody (22503-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 22503-1-AP (same clone as 22503-1-PBS)

    IHC staining of human stomach cancer using 22503-1-AP (same clone as 22503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22503-1-AP (CNOT9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CNOT9 antibody included
  • IHC staining of human stomach cancer using 22503-1-AP (same clone as 22503-1-PBS)

    IHC staining of human stomach cancer using 22503-1-AP (same clone as 22503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22503-1-AP (CNOT9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CNOT9 antibody included
  • IF Staining of hTERT-RPE1 using 22503-1-AP (same clone as 22503-1-PBS)

    IF Staining of hTERT-RPE1 using 22503-1-AP (same clone as 22503-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CNOT9 antibody (22503-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22503-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CNOT9 Ready-To-Use IHC Kit. CNOT9 primary antibody included.

产品信息

22503-1-PBS targets CNOT9 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CNOT9 fusion protein Ag18197 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RCD1 required for cell differentiation1 homolog (S. pombe)
Synonyms
Calculated Molecular Weight 299 aa, 34 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberBC007102
Gene Symbol CNOT9
Gene ID (NCBI) 9125
RRIDAB_11232413
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92600
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RQCD1, also known as CNOT9, is a highly conserved transcriptional cofactor predominantly expressed in actively diffierentiating tissues like testis and thymus. It is a component of the CCR4-NOT complex, the major effector complex in miRNA-mediated gene silencing. Overexpression of RQCD1 has been reported in serveral tumors, indicating that it may be a potential molecular target for cancer treatment.