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KD/KO Validated

CNPY2, MSAP Polyclonal antibody

CNPY2, MSAP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat no : 14635-1-AP

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Synonyms

CNPY2,MSAP, CNPY2, MSAP, MIR-interacting saposin-like protein, HP10390

验证数据展示

WB analysis of mouse pancreas using 14635-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 14635-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 14635-1-AP
IP result of anti-CNPY2, MSAP (IP:14635-1-AP, 3ug; Detection:14635-1-AP 1:500) with HepG2 cells lysate 600ug.
IHC staining of human liver using 14635-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
IF Staining of HepG2 using 14635-1-AP
Immunofluorescent analysis of HepG2 cells using 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 14635-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPY2, MSAP (14635-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB analysis of HepG2 using 14635-1-AP
WB result of CNPY2, MSAP antibody (14635-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells.
IHC staining of human liver using 14635-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
IF Staining of HepG2 using 14635-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNPY2, MSAP antibody (14635-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 14635-1-AP

Size

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经过测试的应用

Positive WB detected in	HepG2 cells, mouse pancreas tissue, human liver tissue, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 4 publications below
WB	See 12 publications below
IHC	See 4 publications below
IF	See 3 publications below

产品信息

14635-1-AP targets CNPY2, MSAP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	CNPY2, MSAP fusion protein Ag6214 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	canopy 2 homolog (zebrafish)
Synonyms	CNPY2,MSAP, CNPY2, MSAP, MIR-interacting saposin-like protein, HP10390
Calculated Molecular Weight	21 kDa
Observed Molecular Weight	17-21 kDa
GenBank Accession Number	BC065015
Gene Symbol	CNPY2
Gene ID (NCBI)	10330
RRID	AB_2082621
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y2B0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2.

实验方案

Product Specific Protocols
WB protocol for CNPY2, MSAP antibody 14635-1-AP	Download protocol
IHC protocol for CNPY2, MSAP antibody 14635-1-AP	Download protocol
IF protocol for CNPY2, MSAP antibody 14635-1-AP	Download protocol
IP protocol for CNPY2, MSAP antibody 14635-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human,mouse	WB	Nat Struct Mol Biol CNPY2 is a key initiator of the PERK-CHOP pathway of the unfolded protein response. Authors - Feng Hong KO Validated View Article
mouse	IHC	Cancers (Basel) Canopy Homolog 2 as a Novel Molecular Target in Hepatocarcinogenesis. Authors - Anna Kakehashi View Article
human	WB,IF	Apoptosis Angiotensin-(1-9) prevents angiotensin II-induced endothelial apoptosis through CNPY2/PERK pathway Authors - Chun-Ling Guo KD Validated View Article
rat	WB	J Cell Mol Med PMID: 31625681 Authors - Lina Zhao View Article
mouse	WB	J Biol Chem Fibroblast growth factor-21 (FGF21) regulates low-density lipoprotein receptor (LDLR) levels in cells via the E3-ubiquitin ligase Mylip/Idol and the Canopy2 (Cnpy2)/Mylip-interacting saposin-like protein (Msap). Authors - Do Hai Thi HT KD Validated View Article
rat	WB	J Biol Chem Reciprocal regulation of Very low-density lipoprotein receptors (VLDLRs) in neurons by Brain-derived neurotrophic factor (BDNF) and Reelin: Involvement of the E3 ligase Mylip/Idol. Authors - Do Hai Thi HT View Article

CNPY2, MSAP Polyclonal antibody

CNPY2, MSAP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

验证数据展示

WB analysis of mouse pancreas using 14635-1-AP

WB analysis using 14635-1-AP

IP experiment of HepG2 using 14635-1-AP

IHC staining of human liver using 14635-1-AP

IF Staining of HepG2 using 14635-1-AP

FC experiment of HepG2 using 14635-1-AP

WB analysis of HepG2 using 14635-1-AP

IHC staining of human liver using 14635-1-AP

IF Staining of HepG2 using 14635-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

CNPY2 is a key initiator of the PERK-CHOP pathway of the unfolded protein response.

Canopy Homolog 2 as a Novel Molecular Target in Hepatocarcinogenesis.

Angiotensin-(1-9) prevents angiotensin II-induced endothelial apoptosis through CNPY2/PERK pathway

PMID: 31625681

Fibroblast growth factor-21 (FGF21) regulates low-density lipoprotein receptor (LDLR) levels in cells via the E3-ubiquitin ligase Mylip/Idol and the Canopy2 (Cnpy2)/Mylip-interacting saposin-like protein (Msap).

Reciprocal regulation of Very low-density lipoprotein receptors (VLDLRs) in neurons by Brain-derived neurotrophic factor (BDNF) and Reelin: Involvement of the E3 ligase Mylip/Idol.

相关产品

Flow Cytometry Perm Buffer (10X)