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  • Featured Product
  • KD/KO Validated

CNPY2, MSAP Monoclonal antibody, PBS Only

CNPY2, MSAP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G8G11

Cat no : 66173-1-PBS

Print datasheet

Synonyms

canopy 2 homolog (zebrafish), CNPY2, CNPY2, MSAP, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9

验证数据展示

  • WB analysis using 66173-1-Ig (same clone as 66173-1-PBS)

    WB analysis using 66173-1-Ig (same clone as 66173-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66173-1-Ig (CNPY2, MSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66173-1-Ig (same clone as 66173-1-PBS)

    IHC staining of human pancreas cancer using 66173-1-Ig (same clone as 66173-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66173-1-Ig (same clone as 66173-1-PBS)

    IHC staining of human pancreas cancer using 66173-1-Ig (same clone as 66173-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66173-1-Ig (CNPY2, MSAP antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66173-1-Ig (same clone as 66173-1-PBS)

    IF Staining of HepG2 using 66173-1-Ig (same clone as 66173-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66173-1-Ig(CNPY2, MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-mouse IgG(H+L). This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66173-1-Ig (same clone as 66173-1-PBS)

    WB analysis of HepG2 using 66173-1-Ig (same clone as 66173-1-PBS)

    WB result of CNPY2, MSAP antibody (66173-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells. This data was developed using the same antibody clone with 66173-1-PBS in a different storage buffer formulation.

产品信息

66173-1-PBS targets CNPY2, MSAP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CNPY2, MSAP fusion protein Ag6363 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name canopy 2 homolog (zebrafish)
Synonyms canopy 2 homolog (zebrafish), CNPY2, CNPY2, MSAP, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 18-21 kDa
GenBank Accession NumberBC065015
Gene Symbol CNPY2
Gene ID (NCBI) 10330
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2B0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2