验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells, human brain tissue, A549 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 2 publications below |
产品信息
15215-1-AP targets CNPY3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | CNPY3 fusion protein Ag7382 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | canopy 3 homolog (zebrafish) |
| 别名 | Expanded repeat-domain protein CAG/CTG 5, ERDA5, CTG4A, CTG repeat protein 4a, canopy 3 homolog (zebrafish) |
| 计算分子量 | 31 kDa |
| 观测分子量 | 30-40 kDa |
| GenBank蛋白编号 | BC004423 |
| 基因名称 | CNPY3 |
| Gene ID (NCBI) | 10695 |
| RRID | AB_11182172 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BT09 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNPY3 antibody 15215-1-AP | Download protocol |
| IHC protocol for CNPY3 antibody 15215-1-AP | Download protocol |
| IF protocol for CNPY3 antibody 15215-1-AP | Download protocol |
| IP protocol for CNPY3 antibody 15215-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Oncol (Dordr) The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells | ||
J Neuroimmunol A novel rare variant of CNPY3 from familial NMOSD impairs the TLR-mediated immune response | ||
World J Gastrointest Oncol Canopy FGF signaling regulator 3 affects prognosis, immune infiltration, and PI3K/AKT pathway in colon adenocarcinoma |
