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  • Featured Product
  • KD/KO Validated

CNPY3 Polyclonal antibody

CNPY3 Polyclonal Antibody for WB, IP, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 15215-1-AP

Print datasheet

Synonyms

CAG4A, canopy 3 homolog (zebrafish), CNPY3, CTG repeat protein 4a, CTG4A, ERDA5, PRAT4A, Protein associated with TLR4, Protein canopy homolog 3, TNRC5, UNQ1934/PRO4409

验证数据展示

  • IHC staining of human colon cancer using 15215-1-AP

    IHC staining of human colon cancer using 15215-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15215-1-AP (CNPY3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse kidney using 15215-1-AP

    IP experiment of mouse kidney using 15215-1-AP

    IP result of anti-CNPY3 (IP:15215-1-AP, 3ug; Detection:15215-1-AP 1:500) with mouse kidney tissue lysate 6000ug.

  • WB analysis of A549 using 15215-1-AP

    WB analysis of A549 using 15215-1-AP

    WB result of CNPY3 antibody (15215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY3 transfected A549 cells.

  • WB analysis using 15215-1-AP

    WB analysis using 15215-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15215-1-AP (CNPY3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 15215-1-AP

    IF Staining of HEK-293 using 15215-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CNPY3 antibody (15215-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, human brain tissue, A549 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15215-1-AP targets CNPY3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CNPY3 fusion protein Ag7382 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name canopy 3 homolog (zebrafish)
Synonyms CAG4A, canopy 3 homolog (zebrafish), CNPY3, CTG repeat protein 4a, CTG4A, ERDA5, PRAT4A, Protein associated with TLR4, Protein canopy homolog 3, TNRC5, UNQ1934/PRO4409
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberBC004423
Gene Symbol CNPY3
Gene ID (NCBI) 10695
RRIDAB_11182172
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BT09
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CNPY3 antibody 15215-1-APDownload protocol
IHC protocol for CNPY3 antibody 15215-1-APDownload protocol
IF protocol for CNPY3 antibody 15215-1-APDownload protocol
IP protocol for CNPY3 antibody 15215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Oncol (Dordr)

The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells

Authors - Yao-Qi Zhou
View Article
humanWB

J Neuroimmunol

A novel rare variant of CNPY3 from familial NMOSD impairs the TLR-mediated immune response

Authors - Yongxin Mo
View Article