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验证数据展示

  • WB analysis using 29708-1-AP

    WB analysis using 29708-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29708-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

Cannabinoid receptor 1 Polyclonal antibody

Cannabinoid receptor 1 Polyclonal Antibody for WB, ELISA
Cat No. 29708-1-AP

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Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, ELISA

Synonyms

CANN6, Cannabinoid receptor 1, cannabinoid receptor 1 (brain), CB R, CB1, CB1A, CB1K5, CB1R, CNR, CNR1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, SH-SY5Y cells, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29708-1-AP targets Cannabinoid receptor 1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse
Immunogen Cannabinoid receptor 1 fusion protein Ag31838 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cannabinoid receptor 1 (brain)
Synonyms CANN6, Cannabinoid receptor 1, cannabinoid receptor 1 (brain), CB R, CB1, CB1A, CB1K5, CB1R, CNR, CNR1
Calculated Molecular Weight 472 aa, 53 kDa
Observed Molecular Weight48 kDa, 66 kDa
GenBank Accession NumberBC074812
Gene Symbol Cannabinoid receptor 1
Gene ID (NCBI) 1268
RRIDAB_2918340
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21554
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cannabinoid receptor 1 (CNR1, or CB1) and CNR2 (CB2) are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. The CB1 receptor is expressed mainly in the brain. The CB2 receptor is expressed mainly in the immune system and in hematopoietic cells. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana.

实验方案

Product Specific Protocols
WB protocol for Cannabinoid receptor 1 antibody 29708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

ACS Chem Neurosci

Basolateral Amygdala Cannabinoid CB1 Receptor Controls Formation and Elimination of Social Fear Memory

Authors - Ming Li
  • KO Validated
View Article