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CNTFR Polyclonal antibody

CNTFR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10796-1-AP

Print datasheet

Synonyms

CNTF receptor subunit alpha, CNTFR, CNTFR alpha

验证数据展示

  • WB analysis using 10796-1-AP

    WB analysis using 10796-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 10796-1-AP

    IHC staining of mouse brain using 10796-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 10796-1-AP

    IHC staining of mouse brain using 10796-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, mouse cerebellum tissue, SH-SY5Y cells, rat brain tissue
Positive IHC detected inmouse brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10796-1-AP targets CNTFR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Immunogen CNTFR fusion protein Ag1189 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ciliary neurotrophic factor receptor
Synonyms CNTF receptor subunit alpha, CNTFR, CNTFR alpha
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC001492
Gene Symbol CNTFR
Gene ID (NCBI) 1271
RRIDAB_2083194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26992
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ciliary neurotrophic factor (CNTF) is a pluripotent neurocytokine. The functional receptor complex of CNTF is composed of the CNTF receptor alpha (CNTFR), gp130 and the leukemia inhibitory factor receptor (LIFR) (PMID: 11943154). Binding of CNTF to CNTFR recruits gp130 and LIFR, which leads to activation of JAK/STAT and Ras/Raf/MAPK pathways.

实验方案

Product Specific Protocols
WB protocol for CNTFR antibody 10796-1-APDownload protocol
IHC protocol for CNTFR antibody 10796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Front Pharmacol

Synergic Neuroprotection Between Ligusticum Chuanxiong Hort and Borneol Against Ischemic Stroke by Neurogenesis via Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier.

Authors - Bin Yu
View Article