验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13843-1-AP targets CNTN1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CNTN1 fusion protein Ag4803 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | contactin 1 |
| 别名 | CNTN1, contactin 1, F3, Glycoprotein gp135, GP135, Neural cell surface protein F3 |
| 计算分子量 | 1018 aa, 113 kDa |
| 观测分子量 | 135 kDa |
| GenBank蛋白编号 | BC036569 |
| 基因名称 | CNTN1 |
| Gene ID (NCBI) | 1272 |
| RRID | AB_2877981 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12860 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNTN1 antibody 13843-1-AP | Download protocol |
| IHC protocol for CNTN1 antibody 13843-1-AP | Download protocol |
| IP protocol for CNTN1 antibody 13843-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.
| ||
Nat Commun Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. | ||
Front Mol Neurosci Contactin-1/F3 Regulates Neuronal Migration and Morphogenesis Through Modulating RhoA Activity.
| ||
Front Genet CNTN-1 Upregulation Induced by Low-Dose Cisplatin Promotes Malignant Progression of Lung Adenocarcinoma Cells via Activation of Epithelial-Mesenchymal Transition. | ||
Arch Med Sci CNTN-1 promotes docetaxel resistance and epithelial-to-mesenchymal transition via the PI3K/Akt signaling pathway in prostate cancer. |