• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67061-1-Ig

    WB analysis using 67061-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67061-1-Ig (CNTROB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 67061-1-Ig

    IF Staining of hTERT-RPE1 using 67061-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CNTROB antibody (67061-1-Ig, Clone: 3D12A4 ) at dilution of 1:350 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

CNTROB Monoclonal antibody

CNTROB Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67061-1-Ig

产品说明书

CloneNo. 3D12A4

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IF/ICC, ELISA

别名

Centrobin, CNTROB, LIP8, LYST interacting protein 8, PP1221

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, pig brain tissue, rat brain tissue, NCI-H1299 cells, JAR cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy CNTROB Ready-To-Use IHC Kit. CNTROB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:175-1:700
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67061-1-Ig targets CNTROB in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 CNTROB fusion protein Ag25447 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 centrobin, centrosomal BRCA2 interacting protein
别名 Centrobin, CNTROB, LIP8, LYST interacting protein 8, PP1221
计算分子量 903 aa, 101 kDa
观测分子量 97 kDa
GenBank蛋白编号BC021134
基因名称 CNTROB
Gene ID (NCBI) 116840
RRIDAB_2882371
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N137
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CNTROB antibody 67061-1-IgDownload protocol
IF protocol for CNTROB antibody 67061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols