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验证数据展示

  • IHC staining of human placenta using 31191-1-AP

    IHC staining of human placenta using 31191-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 31191-1-AP

    IHC staining of human placenta using 31191-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 31191-1-AP

    WB analysis using 31191-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31191-1-AP (COBLL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 31191-1-AP

    IHC staining of mouse kidney using 31191-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31191-1-AP (COBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HaCaT using 31191-1-AP

    IF Staining of HaCaT using 31191-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using COBLL1 antibody (31191-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

COBLL1 Polyclonal antibody

COBLL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31191-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA0977, Cordon bleu protein like 1, COBLR1, COBL like 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inmouse kidney tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31191-1-AP targets COBLL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 COBLL1 fusion protein Ag34853 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 COBL-like 1
别名 KIAA0977, Cordon bleu protein like 1, COBLR1, COBL like 1
观测分子量160 kDa
GenBank蛋白编号BC071588
基因名称 COBLL1
Gene ID (NCBI) 22837
RRIDAB_3669891
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ53SF7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cordon-blue protein-like 1 (COBLL1), a negative regulator of apoptosis, is associated with lower insulin resistance. COBLL1 is involved in the cancer cell morphogenesis to a neuron-like cell shape observed in the CRPC model cells, promoting cell growth and migration (PMID: 29686105, 29686105). COBLL1 is highly expressed in most human tissues including lung, liver, kidney, pancreas, ovary, spinal cord and brain.

实验方案

Product Specific Protocols
WB protocol for COBLL1 antibody 31191-1-APDownload protocol
IHC protocol for COBLL1 antibody 31191-1-APDownload protocol
IF protocol for COBLL1 antibody 31191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols