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验证数据展示

  • WB analysis of human brain using 14277-1-AP

    WB analysis of human brain using 14277-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 14277-1-AP

    WB analysis using 14277-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 14277-1-AP

    IF Staining of MCF-7 using 14277-1-AP

    Immunofluorescent analysis of MCF-7 cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human stomach cancer using 14277-1-AP

    IHC staining of human stomach cancer using 14277-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14277-1-AP (COG6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 14277-1-AP

    IHC staining of human stomach cancer using 14277-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14277-1-AP (COG6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COG6 Polyclonal antibody

COG6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14277-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA1134, COG complex subunit 6, COD2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, Hela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14277-1-AP targets COG6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 COG6 fusion protein Ag5624 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 component of oligomeric golgi complex 6
别名 KIAA1134, COG complex subunit 6, COD2
计算分子量 73 kDa
观测分子量 73 kDa
GenBank蛋白编号BC051723
基因名称 COG6
Gene ID (NCBI) 57511
RRIDAB_10694426
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2V7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COG6 antibody 14277-1-APDownload protocol
IHC protocol for COG6 antibody 14277-1-APDownload protocol
IF protocol for COG6 antibody 14277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols