• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

COG6 Polyclonal antibody

COG6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14277-1-AP

Print datasheet

Synonyms

KIAA1134, COG complex subunit 6, COD2

验证数据展示

  • WB analysis of human brain using 14277-1-AP

    WB analysis of human brain using 14277-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 14277-1-AP

    WB analysis using 14277-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14277-1-AP (COG6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 14277-1-AP

    IF Staining of MCF-7 using 14277-1-AP

    Immunofluorescent analysis of MCF-7 cells, using COG6 antibody 14277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human stomach cancer using 14277-1-AP

    IHC staining of human stomach cancer using 14277-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14277-1-AP (COG6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 14277-1-AP

    IHC staining of human stomach cancer using 14277-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 14277-1-AP (COG6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue, HEK-293 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14277-1-AP targets COG6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen COG6 fusion protein Ag5624 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name component of oligomeric golgi complex 6
Synonyms KIAA1134, COG complex subunit 6, COD2
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC051723
Gene Symbol COG6
Gene ID (NCBI) 57511
RRIDAB_10694426
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2V7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COG6 antibody 14277-1-APDownload protocol
IHC protocol for COG6 antibody 14277-1-APDownload protocol
IF protocol for COG6 antibody 14277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols