验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67218-1-Ig targets COIL in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | Human |
免疫原 | COIL fusion protein Ag17171 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | coilin |
别名 | Coilin, p80-coilin, p80 coilin, CLN80, 2G12B8 |
计算分子量 | 576 aa, 63 kDa |
观测分子量 | 80 kDa |
GenBank蛋白编号 | BC010385 |
基因名称 | COIL |
Gene ID (NCBI) | 8161 |
RRID | AB_2882509 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P38432 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for COIL antibody 67218-1-Ig | Download protocol |
IF protocol for COIL antibody 67218-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |