验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, SKOV-3 cells, T-47D cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51113-1-AP targets COL12A1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | collagen, type XII, alpha 1 |
| 别名 | COL12, Collagen Type XII, Collagen alpha-1(XII) chain, Collagen alpha 1(XII) chain, COL12A1L |
| 计算分子量 | 333 kDa |
| 观测分子量 | 333-460 kDa |
| GenBank蛋白编号 | NM_004370 |
| 基因名称 | COL12A1 |
| Gene ID (NCBI) | 1303 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99715 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COL12A1 (Collagen alpha-1(XII) chain), also known as COL12A1L. It is predicted to be located in the ECM. Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix. The molecular weight of COL12A1 is 333 kDa. It is reported that the molecular weight of the protein is 268-460kDa (PMID: 31273343).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COL12A1 antibody 51113-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |