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验证数据展示

  • WB analysis using 31190-1-AP

    WB analysis using 31190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31190-1-AP (COL17A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat skin using 31190-1-AP

    IHC staining of rat skin using 31190-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skin using 31190-1-AP

    IHC staining of rat skin using 31190-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 31190-1-AP

    IF Staining of A431 using 31190-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HaCaT using 31190-1-AP

    IF Staining of HaCaT using 31190-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Collagen Type XVII Polyclonal antibody

Collagen Type XVII Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31190-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

COL17, COL17A1, LABD97, BP180, Collagen alpha 1(XVII) chain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, mouse skin tissue
Positive IHC detected inrat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HaCaT cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

31190-1-AP targets Collagen Type XVII in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 Collagen Type XVII fusion protein Ag34645 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type XVII, alpha 1
别名 COL17, COL17A1, LABD97, BP180, Collagen alpha 1(XVII) chain
观测分子量180 kDa
GenBank蛋白编号BC168368
基因名称 COL17A1
Gene ID (NCBI) 1308
RRIDAB_3669890
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9UMD9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collagen alpha-1(XVII) chain, also termed 180-kDa bullous pemphigoid antigen (BP180), is a type II transmembrane glycoprotein with a collagenous carboxyl-terminal extracellular domain and a noncollagenous amino-terminal cytoplasmic domain. High expression of COL17A1 has also been identified as a marker of human esophageal quiescent stem/progenitor cells (PMID: 35709398, 9545306). As a structural component of the hemidesmosome, COL17A1 is not only present as the full-length 180 kDa transmembrane form, but also as a cleaved 120 kDa soluble form that is further processed to a 97 kDa form (PMID: 113594550).

实验方案

Product Specific Protocols
WB protocol for Collagen Type XVII antibody 31190-1-APDownload protocol
IHC protocol for Collagen Type XVII antibody 31190-1-APDownload protocol
IF protocol for Collagen Type XVII antibody 31190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Invest Dermatol

Paxbp1 is indispensable for the maintenance of epidermal homeostasis

Authors - Cong Huang
View Article