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COL17A1 Polyclonal antibody

COL17A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31190-1-AP

Print datasheet

Synonyms

97 kDa LAD antigen, BP180, BPAG2, Bullous pemphigoid antigen 2, COL17A1, Collagen alpha 1(XVII) chain, collagen, type XVII, alpha 1, FLJ60881, KIAA0204, LABD97, LAD 1, Linear IgA disease antigen 1

验证数据展示

  • WB analysis using 31190-1-AP

    WB analysis using 31190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31190-1-AP (COL17A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat skin using 31190-1-AP

    IHC staining of rat skin using 31190-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skin using 31190-1-AP

    IHC staining of rat skin using 31190-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 31190-1-AP

    IF Staining of A431 using 31190-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HaCaT using 31190-1-AP

    IF Staining of HaCaT using 31190-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA431 cells, mouse skin tissue
Positive IHC detected inrat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HaCaT cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31190-1-AP targets COL17A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen COL17A1 fusion protein Ag34645 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type XVII, alpha 1
Synonyms 97 kDa LAD antigen, BP180, BPAG2, Bullous pemphigoid antigen 2, COL17A1, Collagen alpha 1(XVII) chain, collagen, type XVII, alpha 1, FLJ60881, KIAA0204, LABD97, LAD 1, Linear IgA disease antigen 1
Observed Molecular Weight180 kDa
GenBank Accession NumberBC168368
Gene Symbol COL17A1
Gene ID (NCBI) 1308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UMD9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collagen alpha-1(XVII) chain, also termed 180-kDa bullous pemphigoid antigen (BP180), is a type II transmembrane glycoprotein with a collagenous carboxyl-terminal extracellular domain and a noncollagenous amino-terminal cytoplasmic domain. High expression of COL17A1 has also been identified as a marker of human esophageal quiescent stem/progenitor cells (PMID: 35709398, 9545306). As a structural component of the hemidesmosome, COL17A1 is not only present as the full-length 180 kDa transmembrane form, but also as a cleaved 120 kDa soluble form that is further processed to a 97 kDa form (PMID: 113594550).

实验方案

Product Specific Protocols
WB protocol for COL17A1 antibody 31190-1-APDownload protocol
IHC protocol for COL17A1 antibody 31190-1-APDownload protocol
IF protocol for COL17A1 antibody 31190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols