验证数据展示

WB analysis of pig skin using 14695-1-AP
pig skin tissue were subjected to SDS PAGE followed by western blot with 14695-1-AP (Collagen Type I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human skin cancer using 14695-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14695-1-AP (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 14695-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 14695-1-AP (Collagen Type I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB analysis using 14695-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14695-1-AP (Collagen Type I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 14695-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14695-1-AP (Collagen Type I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 14695-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14695-1-AP (Collagen Type I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of U-87 MG using 14695-1-AP
IP result of anti-Collagen Type I (IP:14695-1-AP, 4ug; Detection:14695-1-AP 1:2000) with U-87 MG cells lysate 1920 ug.
IHC staining of human colon using 14695-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14695-1-AP (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 14695-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14695-1-AP (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 14695-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14695-1-AP (Collagen Type I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of SW480 using 14695-1-AP
1X10^6 SW480 cells were intracellularly stained with 0.2 ug Anti-Human Collagen Type I (14695-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Collagen Type I Polyclonal antibody

Collagen Type I Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 14695-1-AP

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Publications(1054)

Host / Isotype

Rabbit / IgG

Reactivity

human, pig and More (7)

Applications

WB, IHC, FC (Intra), IP, ELISA and More (2)

Synonyms

COL1A2, COL1, collagen, type I, alpha 2, Collagen alpha 2(I) chain, col I

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size:

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概述

经过测试的应用
产品信息
发表文章 (1054)
实验方案

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MSDS

经过测试的应用

Positive WB detected in	pig skin tissue, human placenta tissue, MG-63 cells, Saos-2 cells, U-87 MG cells
Positive IP detected in	U-87 MG cells
Positive IHC detected in	human skin cancer tissue, human colon tissue, human oesophagus cancer tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	SW480 cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 846 publications below
IHC	See 222 publications below
IF	See 190 publications below
IP	See 1 publications below
FC	See 2 publications below
CoIP	See 1 publications below

产品信息

14695-1-AP targets Collagen Type I in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, pig samples.

Tested Applications	WB, IHC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, FC (Intra), IP, CoIP
Tested Reactivity	human, pig
Cited Reactivity	human, mouse, rat, pig, rabbit, canine, chicken, bovine, toad
Immunogen	Collagen Type I fusion protein Ag6281 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	collagen, type I, alpha 2
Synonyms	COL1A2, COL1, collagen, type I, alpha 2, Collagen alpha 2(I) chain, col I
Calculated Molecular Weight	1366 aa, 130 kDa
Observed Molecular Weight	130-150 kDa
GenBank Accession Number	BC054498
Gene Symbol	COL1A2
Gene ID (NCBI)	1278
RRID	AB_2082037
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08123
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

实验方案

Product Specific Protocols
WB protocol for Collagen Type I antibody 14695-1-AP	Download protocol
IHC protocol for Collagen Type I antibody 14695-1-AP	Download protocol
IP protocol for Collagen Type I antibody 14695-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Cancer Cell Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis Authors - Jiang Chang View Article
rat	IF	Adv Mater Acid-Induced in Situ Phase Separation and Percolation for Constructing Bi-Continuous Phase Hydrogel Electrodes With Motion-Insensitive Property Authors - Qingquan Han View Article
mouse	WB	Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells Authors - Decai Wang View Article
rabbit	IF	Adv Mater A Superparamagnetic Composite Hydrogel Scaffold as in Vivo Dynamic Monitorable Theranostic Platform for Osteoarthritis Regeneration Authors - Mayifei Rong View Article
mouse	WB	Gut Histone methyltransferase Suv39h1 regulates hepatic stellate cell activation and is targetable in liver fibrosis Authors - Ming Kong View Article
human	WB	Bioact Mater 3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration Authors - Lei Zhang View Article

验证数据展示

WB analysis of pig skin using 14695-1-AP

IHC staining of human skin cancer using 14695-1-AP

IHC staining of human skin cancer using 14695-1-AP

WB analysis using 14695-1-AP

WB analysis using 14695-1-AP

WB analysis using 14695-1-AP

IP experiment of U-87 MG using 14695-1-AP

IHC staining of human colon using 14695-1-AP

IHC staining of human colon using 14695-1-AP

IHC staining of human placenta using 14695-1-AP

FC experiment of SW480 using 14695-1-AP

Collagen Type I Polyclonal antibody

Collagen Type I Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

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经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis

Acid-Induced in Situ Phase Separation and Percolation for Constructing Bi-Continuous Phase Hydrogel Electrodes With Motion-Insensitive Property

Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells

A Superparamagnetic Composite Hydrogel Scaffold as in Vivo Dynamic Monitorable Theranostic Platform for Osteoarthritis Regeneration

Histone methyltransferase Suv39h1 regulates hepatic stellate cell activation and is targetable in liver fibrosis

3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration