Collagen Type I Polyclonal antibody

Collagen Type I Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Cat No. 14695-1-AP

产品说明书

引用文献(1064)

宿主/亚型

Rabbit / IgG

种属反应性

human, pig and More (7)

应用

WB, IHC, FC (Intra), IP, ELISA and More (2)

COL1, COL1A2, Alpha-2 type I collagen, col I, Collagen alpha 2(I) chain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inpig skin tissue, human placenta tissue, MG-63 cells, Saos-2 cells, U-87 MG cells
Positive IP detected inU-87 MG cells
Positive IHC detected inhuman skin cancer tissue, human colon tissue, human oesophagus cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inSW480 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14695-1-AP targets Collagen Type I in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, FC (Intra), IP, CoIP
经测试反应性 human, pig
文献引用反应性human, mouse, rat, pig, rabbit, canine, chicken, bovine, toad
免疫原 Collagen Type I fusion protein Ag6281 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type I, alpha 2
别名 COL1, COL1A2, Alpha-2 type I collagen, col I, Collagen alpha 2(I) chain
计算分子量 1366 aa, 130 kDa
观测分子量130-150 kDa
GenBank蛋白编号BC054498
基因名称 COL1A2
Gene ID (NCBI) 1278
RRIDAB_2082037
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08123
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type l collagen can recognize collagen alpha 2(1) chain and C-terminal propeptide of pro-alpha 2(1) chain.

实验方案

Product Specific Protocols
WB protocol for Collagen Type I antibody 14695-1-APDownload protocol
IHC protocol for Collagen Type I antibody 14695-1-APDownload protocol
IP protocol for Collagen Type I antibody 14695-1-APDownload protocol
FC protocol for Collagen Type I antibody 14695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

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