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  • Featured Product
  • KD/KO Validated

Collagen Type XXIII Polyclonal antibody

Collagen Type XXIII Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14337-1-AP

Print datasheet

Synonyms

COL23A1, Collagen alpha 1(XXIII) chain, Collagen Type XXIII, collagen, type XXIII, alpha 1

验证数据展示

  • WB analysis of mouse thymus using 14337-1-AP

    WB analysis of mouse thymus using 14337-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14337-1-AP (Collagen Type XXIII antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 14337-1-AP

    IHC staining of human colon using 14337-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14337-1-AP (Collagen Type XXIII antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 14337-1-AP

    IHC staining of human colon using 14337-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14337-1-AP (Collagen Type XXIII antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse thymus tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14337-1-AP targets Collagen Type XXIII in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Collagen Type XXIII fusion protein Ag5640 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type XXIII, alpha 1
Synonyms COL23A1, Collagen alpha 1(XXIII) chain, Collagen Type XXIII, collagen, type XXIII, alpha 1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 68-75 kDa
GenBank Accession NumberBC042428
Gene Symbol COL23A1
Gene ID (NCBI) 91522
RRIDAB_2276392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86Y22
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Collagen Type XXIII antibody 14337-1-APDownload protocol
IHC protocol for Collagen Type XXIII antibody 14337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

The Oncogenic Role of COL23A1 in Clear Cell Renal Cell Carcinoma.

Authors - Fujiang Xu
  • KD Validated
View Article