• Featured Product
  • KD/KO Validated

Collagen Type III (C-terminal) Polyclonal antibody

Collagen Type III (C-terminal) Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 13548-1-AP

Print datasheet

Synonyms

Collagen Type III (C-terminal)



经过测试的应用

Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COL3A1 Ready-To-Use IHC Kit. COL3A1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13548-1-AP targets Collagen Type III (C-terminal) in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Immunogen Collagen Type III (C-terminal) fusion protein Ag4229 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type III, alpha 1
Synonyms Collagen Type III (C-terminal)
Calculated Molecular Weight 1466 aa, 139 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC028178
Gene Symbol COL3A1
Gene ID (NCBI) 1281
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02461
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Type III collagen is a fibrillar forming collagen comprising three a1(III) chains and is expressed in early embryos and throughout embryogenesis. In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen. COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with type III and IV Ehlers-Danlos syndrome, and with aortic and arterial aneurysms. (PMID: 9050868; 2445760)

实验方案

Product Specific Protocols
IHC protocol for Collagen Type III (C-terminal) antibody 13548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Redox Biol

Silencing COX-2 blocks PDK1/TRAF4-induced AKT activation to inhibit fibrogenesis during skeletal muscle atrophy.

Authors - Hongtao Chen
mouseIHC

Oxid Med Cell Longev

Myricetin Possesses Potential Protective Effects on Diabetic Cardiomyopathy through Inhibiting IκBα/NFκB and Enhancing Nrf2/HO-1.

Authors - Hai-Han Liao
humanWB,IF

Oncotarget

Epithelial but not stromal expression of collagen alpha-1(III) is a diagnostic and prognostic indicator of colorectal carcinoma.

Authors - Xiao-Qing Wang
  • KD Validated

Cell Death Dis

Rosmarinic acid attenuates cardiac fibrosis following long-term pressure overload via AMPKα/Smad3 signaling.

Authors - Xin Zhang
mouse,ratWB

J Mol Cell Cardiol

Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.

Authors - Randy T Cowling
mouseWB

Sci Rep

Deletion of interleukin-6 alleviated interstitial fibrosis in streptozotocin-induced diabetic cardiomyopathy of mice through affecting TGFβ1 and miR-29 pathways.

Authors - Yang Zhang