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COL3A1 Monoclonal antibody, PBS Only

COL3A1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B1F3

Cat no : 68320-1-PBS

Print datasheet

Synonyms

COL3, 3B1F3, collagen, type III, alpha 1, Collagen Type III, Collagen alpha-1(III) chain

验证数据展示

  • WB analysis using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis using 68320-1-Ig (same clone as 68320-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • WB analysis of human rectum using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis of human rectum using 68320-1-Ig (same clone as 68320-1-PBS)

    human rectum tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • WB analysis of rabbit large intestine using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis of rabbit large intestine using 68320-1-Ig (same clone as 68320-1-PBS)

    rabbit large intestine tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human ovary tumor using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of mouse skin using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of mouse skin using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IF Staining of MCF-7 using 68320-1-Ig (same clone as 68320-1-PBS)

    IF Staining of MCF-7 using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using COL3A1 antibody (68320-1-Ig, Clone: 3B1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy COL3A1 Ready-To-Use IHC Kit. COL3A1 primary antibody included.

产品信息

68320-1-PBS targets COL3A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen COL3A1 fusion protein Ag4229 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name collagen, type III, alpha 1
Synonyms COL3, 3B1F3, collagen, type III, alpha 1, Collagen Type III, Collagen alpha-1(III) chain
Calculated Molecular Weight 1466 aa, 139 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC028178
Gene Symbol COL3A1
Gene ID (NCBI) 1281
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02461
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Type III collagen is a fibrillar-forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341).