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Species Reactivity
0
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验证数据展示

  • WB analysis using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis using 68320-1-Ig (same clone as 68320-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • WB analysis of human rectum using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis of human rectum using 68320-1-Ig (same clone as 68320-1-PBS)

    human rectum tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • WB analysis of rabbit large intestine using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis of rabbit large intestine using 68320-1-Ig (same clone as 68320-1-PBS)

    rabbit large intestine tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human ovary tumor using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of mouse skin using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of mouse skin using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IF Staining of MCF-7 using 68320-1-Ig (same clone as 68320-1-PBS)

    IF Staining of MCF-7 using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using COL3A1 antibody (68320-1-Ig, Clone: 3B1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

Collagen Type III Monoclonal antibody, PBS Only

Collagen Type III Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68320-1-PBS

产品说明书

CloneNo. 3B1F3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, Indirect ELISA

别名

COL3, COL3A1, 3B1F3, Collagen alpha 1(III) chain, Collagen alpha-1(III) chain

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy COL3A1 Ready-To-Use IHC Kit. COL3A1 primary antibody included.

产品信息

68320-1-PBS targets Collagen Type III in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Collagen Type III fusion protein Ag4229 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 collagen, type III, alpha 1
别名 COL3, COL3A1, 3B1F3, Collagen alpha 1(III) chain, Collagen alpha-1(III) chain
计算分子量 1466 aa, 139 kDa
观测分子量130 kDa
GenBank蛋白编号BC028178
基因名称 COL3A1
Gene ID (NCBI) 1281
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02461
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Type III collagen is a fibrillar-forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341).