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COL6A3-Specific Polyclonal antibody

COL6A3-Specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 19798-1-AP

Print datasheet

Synonyms

COL6A3, Collagen Type VI, Collagen alpha-3(VI) chain, Collagen alpha 3(VI) chain

验证数据展示

  • IHC staining of human cervical cancer using 19798-1-AP

    IHC staining of human cervical cancer using 19798-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19798-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 19798-1-AP

    IHC staining of human lung cancer using 19798-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19798-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human urothelial carcinoma using 19798-1-AP

    IHC staining of human urothelial carcinoma using 19798-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 19798-1-AP (Collagen Type VI antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman lung cancer tissue, human cervical cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19798-1-AP targets COL6A3-Specific in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type VI, alpha 3
Synonyms COL6A3, Collagen Type VI, Collagen alpha-3(VI) chain, Collagen alpha 3(VI) chain
Calculated Molecular Weight 344 kDa
Observed Molecular Weight343 kDa
GenBank Accession NumberNM_004369
Gene Symbol COL6A3
Gene ID (NCBI) 1293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12111
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COL6A3 belongs to the type VI collagen family. Collagen VI acts as a cell-binding protein. It is expected to be expressed in smooth muscle. And the calculated molecular weight of COL6A3 is 343 kDa. The protein has similar expression in obese and T2DM (2 patients, and it regulates the chemotaxis and inflammation of macrophages in adipose tissue. Its expression is also related to weight gain. The expression of this protein in adipocytes is related to insulin resistance, which is believed to depend on PPAR (peroxisome proliferator-activated receptor) α-mediated adipocyte development (PMID: 25337653). Defects in COL6A3 are a cause of Bethlem myopathy (BM). Defects in COL6A3 are a cause of Ullrich congenital muscular dystrophy (UCMD) which also known as Ullrich scleroatonic muscular dystrophy. This antibody is specific to COL6A3.

实验方案

Product Specific Protocols
IHC protocol for COL6A3-Specific antibody 19798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols