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验证数据展示

  • IHC staining of mouse lung using 22302-1-AP

    IHC staining of mouse lung using 22302-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22302-1-AP (COL6A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 22302-1-AP

    WB analysis using 22302-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22302-1-AP (COL6A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 22302-1-AP

    IHC staining of mouse heart using 22302-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22302-1-AP (COL6A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COL6A6 Polyclonal antibody

COL6A6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22302-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, Mouse and More (1)

应用

WB, IHC, ELISA

别名

COL6A6, Collagen alpha 6(VI) chain, collagen type VI alpha 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

22302-1-AP targets COL6A6 in WB, IHC, ELISA applications and shows reactivity with human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, Mouse
文献引用反应性mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen type VI alpha 6
别名 COL6A6, Collagen alpha 6(VI) chain, collagen type VI alpha 6
计算分子量 247 kDa
观测分子量167 kDa, 250 kDa
GenBank蛋白编号NM_001102608
基因名称 COL6A6
Gene ID (NCBI) 131873
RRIDAB_3085695
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA6NMZ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collagen type VI alpha 6 chain (COL6A6) belongs to the collagen VI (COL6) family which acts as a vital role in the extracellular matrix. COL6A6 encodes a 2,262-amino acid protein that contains multiple von Willebrand factor domains and forms a component of the basal lamina of epithelial cells (PMID: 33747955). COL6A6 is a novel tumor suppressor gene in NSCLC and can serve as a potentially valuable prognostic biomarker and therapeutic target for NSCLC (PMID: 35116307).

实验方案

Product Specific Protocols
WB protocol for COL6A6 antibody 22302-1-APDownload protocol
IHC protocol for COL6A6 antibody 22302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Agric Food Chem

Combination of Structural Analysis and Proteomics Strategy Revealed the Mechanism of Ultrasound-Assisted Cold Plasma Regulating Shrimp Allergy

Authors - Hangyu Zhong
View Article