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COL6A6 Polyclonal antibody

COL6A6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22302-1-AP

Print datasheet

Synonyms

COL6A6, Collagen alpha 6(VI) chain, collagen type VI alpha 6

验证数据展示

  • IHC staining of mouse lung using 22302-1-AP

    IHC staining of mouse lung using 22302-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22302-1-AP (COL6A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 22302-1-AP

    WB analysis using 22302-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22302-1-AP (COL6A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 22302-1-AP

    IHC staining of mouse heart using 22302-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22302-1-AP (COL6A6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

22302-1-AP targets COL6A6 in WB, IHC, ELISA applications and shows reactivity with human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, Mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen type VI alpha 6
Synonyms COL6A6, Collagen alpha 6(VI) chain, collagen type VI alpha 6
Calculated Molecular Weight 247 kDa
Observed Molecular Weight167 kDa, 250 kDa
GenBank Accession NumberNM_001102608
Gene Symbol COL6A6
Gene ID (NCBI) 131873
RRIDAB_3085695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NMZ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collagen type VI alpha 6 chain (COL6A6) belongs to the collagen VI (COL6) family which acts as a vital role in the extracellular matrix. COL6A6 encodes a 2,262-amino acid protein that contains multiple von Willebrand factor domains and forms a component of the basal lamina of epithelial cells (PMID: 33747955). COL6A6 is a novel tumor suppressor gene in NSCLC and can serve as a potentially valuable prognostic biomarker and therapeutic target for NSCLC (PMID: 35116307).

实验方案

Product Specific Protocols
WB protocol for COL6A6 antibody 22302-1-APDownload protocol
IHC protocol for COL6A6 antibody 22302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Agric Food Chem

Combination of Structural Analysis and Proteomics Strategy Revealed the Mechanism of Ultrasound-Assisted Cold Plasma Regulating Shrimp Allergy

Authors - Hangyu Zhong
View Article