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Collagen Type VII Polyclonal antibody

Collagen Type VII Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19799-1-AP

Print datasheet

Synonyms

COL7A1, Collagen alpha 1(VII) chain, Collagen Type VII, collagen, type VII, alpha 1, EBD1, EBDCT, EBR1, LC collagen, Long chain collagen

验证数据展示

  • WB analysis using 19799-1-AP

    WB analysis using 19799-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19799-1-AP (Collagen Type VII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19799-1-AP targets Collagen Type VII in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type VII, alpha 1
Synonyms COL7A1, Collagen alpha 1(VII) chain, Collagen Type VII, collagen, type VII, alpha 1, EBD1, EBDCT, EBR1, LC collagen, Long chain collagen
Calculated Molecular Weight 295 kDa
Observed Molecular Weight 290 kDa
GenBank Accession NumberNM_000094
Gene Symbol COL7A1
Gene ID (NCBI) 1294
RRIDAB_2878609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02388
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COL7A1, also named as LC collagen, is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica (DEB). Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica Pasini type (P-DEB) which also known as albopapuloid dominant dystrophic epidermolysis bullosa. Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica Hallopeau-Siemens type (HS-DEB). Defects in COL7A1 are the cause of transient bullous dermolysis of the newborn (TBDN). Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica pretibial type (PR-DEB). Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica Bart type (B-DEB). Defects in COL7A1 are the cause of epidermolysis bullosa pruriginosa (EBP). Defects in COL7A1 are the cause of isolated toenail dystrophy without skin fragility. Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica with subcorneal cleavage (EBDSC) which also known as epidermolysis bullosa simplex superficialis (EBSS). This antibody is specific to COL7A1.

实验方案

Product Specific Protocols
WB protocol for Collagen Type VII antibody 19799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols