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验证数据展示

  • WB analysis using 19799-1-AP

    WB analysis using 19799-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19799-1-AP (Collagen Type VII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Collagen Type VII Polyclonal antibody

Collagen Type VII Polyclonal Antibody for WB, ELISA
Cat No. 19799-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

COL7A1, Collagen alpha 1(VII) chain, Collagen Type VII, collagen, type VII, alpha 1, EBD1, EBDCT, EBR1, LC collagen, Long chain collagen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19799-1-AP targets Collagen Type VII in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type VII, alpha 1
别名 COL7A1, Collagen alpha 1(VII) chain, Collagen Type VII, collagen, type VII, alpha 1, EBD1, EBDCT, EBR1, LC collagen, Long chain collagen
计算分子量 295 kDa
观测分子量 290 kDa
GenBank蛋白编号NM_000094
基因名称 COL7A1
Gene ID (NCBI) 1294
RRIDAB_2878609
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02388
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COL7A1, also named as LC collagen, is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica (DEB). Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica Pasini type (P-DEB) which also known as albopapuloid dominant dystrophic epidermolysis bullosa. Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica Hallopeau-Siemens type (HS-DEB). Defects in COL7A1 are the cause of transient bullous dermolysis of the newborn (TBDN). Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica pretibial type (PR-DEB). Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica Bart type (B-DEB). Defects in COL7A1 are the cause of epidermolysis bullosa pruriginosa (EBP). Defects in COL7A1 are the cause of isolated toenail dystrophy without skin fragility. Defects in COL7A1 are the cause of epidermolysis bullosa dystrophica with subcorneal cleavage (EBDSC) which also known as epidermolysis bullosa simplex superficialis (EBSS). This antibody is specific to COL7A1.

实验方案

Product Specific Protocols
WB protocol for Collagen Type VII antibody 19799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols