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验证数据展示

  • WB analysis using 12507-1-AP

    WB analysis using 12507-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12507-1-AP (Collagen Type IX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

COL9A1 Polyclonal antibody

COL9A1 Polyclonal Antibody for WB, ELISA
Cat No. 12507-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

COL9, Collagen alpha 1(IX) chain, Collagen alpha-1(IX) chain, Collagen Type IX, EDM6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12507-1-AP targets COL9A1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 COL9A1 fusion protein Ag3189 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type IX, alpha 1
别名 COL9, Collagen alpha 1(IX) chain, Collagen alpha-1(IX) chain, Collagen Type IX, EDM6
计算分子量 921 aa 92 kDa
观测分子量64-67 kDa
GenBank蛋白编号BC015409
基因名称 COL9A1
Gene ID (NCBI) 1297
RRIDAB_3669142
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20849
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COL9A1 (collagen type IX alpha 1 chain), also known as MED. It is expected to be located in extracellular space, and the protein is enriched in the brain, choroid plexus and prostate. This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. The molecular weight of COL9A1 is 64 kDa, and it can recognize other isoforms.

实验方案

Product Specific Protocols
WB protocol for COL9A1 antibody 12507-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols