验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, human heart tissue, human stomach tissue, mouse stomach tissue, mouse heart tissue, HEK-293 cells, MCF-7 cells, human placenta tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10393-1-AP targets COMMD5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | COMMD5 fusion protein Ag0592 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | COMM domain containing 5 |
| 别名 | Hypertension-related calcium-regulated gene protein, HT002, HCaRG, COMM domain-containing protein 5, COMM domain containing 5 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC003055 |
| 基因名称 | COMMD5 |
| Gene ID (NCBI) | 28991 |
| RRID | AB_2083555 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9GZQ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COMMD5 antibody 10393-1-AP | Download protocol |
| IF protocol for COMMD5 antibody 10393-1-AP | Download protocol |
| IP protocol for COMMD5 antibody 10393-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.
| ||
Elife Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes. | ||
Mol Metab Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion. | ||
Am J Physiol Renal Physiol COMMD5 counteracts cisplatin-induced nephrotoxicity by maintaining tubular epithelial integrity and autophagy flux | ||
