COMT Polyclonal antibody

COMT Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 14754-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

catechol O methyltransferase, Catechol O-methyltransferase, EC:2.1.1.6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, human placenta, mouse liver, rat liver
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver tissue, human placenta tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14754-1-AP targets COMT in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, monkey
免疫原 COMT fusion protein Ag6502 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 catechol-O-methyltransferase
别名 catechol O methyltransferase, Catechol O-methyltransferase, EC:2.1.1.6
计算分子量 30 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC000419
基因名称 COMT
Gene ID (NCBI) 1312
RRIDAB_2083706
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21964
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

实验方案

Product Specific Protocols
WB protocol for COMT antibody 14754-1-APDownload protocol
IHC protocol for COMT antibody 14754-1-APDownload protocol
IF protocol for COMT antibody 14754-1-APDownload protocol
IP protocol for COMT antibody 14754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.

Authors - Xiao Wu
mouseWB

Aging (Albany NY)

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.

Authors - Dongdong Wang
mouseWB

Transl Psychiatry

A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.

Authors - Yinrui Guo
mouseWB

FASEB J

Entacapone alleviates acute kidney injury by inhibiting ferroptosis.

Authors - Jiahong Yang
humanWB,IHC

Endocrinology

Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells.

Authors - Saller S S
humanIHC

J Proteomics

Proteomic analysis of podocyte exosome-enriched fraction from normal human urine.

Authors - Prunotto Marco M
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