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验证数据展示

  • WB analysis using 27469-1-AP

    WB analysis using 27469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27469-1-AP (COPB1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 27469-1-AP

    IHC staining of human stomach using 27469-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27469-1-AP (COPB1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPB1 antibody included

COPB1 Polyclonal antibody

COPB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27469-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

COPB, Coatomer subunit beta, Beta-COP, Beta-coat protein, Beta COP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COPB1 Ready-To-Use IHC Kit. COPB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27469-1-AP targets COPB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 COPB1 fusion protein Ag26518 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coatomer protein complex, subunit beta 1
别名 COPB, Coatomer subunit beta, Beta-COP, Beta-coat protein, Beta COP
计算分子量 107 kDa
观测分子量 95-100 kDa
GenBank蛋白编号BC037280
基因名称 COPB1
Gene ID (NCBI) 1315
RRIDAB_2880880
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53618
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COPB1 antibody 27469-1-APDownload protocol
IHC protocol for COPB1 antibody 27469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols