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COPB1 Polyclonal antibody

COPB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27469-1-AP

Print datasheet

Synonyms

COPB, Coatomer subunit beta, Beta-COP, Beta-coat protein, Beta COP

验证数据展示

  • WB analysis using 27469-1-AP

    WB analysis using 27469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27469-1-AP (COPB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 27469-1-AP

    IHC staining of human stomach using 27469-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27469-1-AP (COPB1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPB1 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COPB1 Ready-To-Use IHC Kit. COPB1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27469-1-AP targets COPB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen COPB1 fusion protein Ag26518 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coatomer protein complex, subunit beta 1
Synonyms COPB, Coatomer subunit beta, Beta-COP, Beta-coat protein, Beta COP
Calculated Molecular Weight 107 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC037280
Gene Symbol COPB1
Gene ID (NCBI) 1315
RRIDAB_2880880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53618
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COPB1 antibody 27469-1-APDownload protocol
IHC protocol for COPB1 antibody 27469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols