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COPB2 Polyclonal antibody

COPB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14733-1-AP

Print datasheet

Synonyms

p102, Coatomer subunit beta', Beta'-COP, Beta'-coat protein

验证数据展示

  • WB analysis using 14733-1-AP

    WB analysis using 14733-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14733-1-AP (COPB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 14733-1-AP

    IHC staining of human stomach using 14733-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14733-1-AP (COPB2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPB2 antibody included
  • IF Staining of PC-12 using 14733-1-AP

    IF Staining of PC-12 using 14733-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using COPB2 antibody (14733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman stomach tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy COPB2 Ready-To-Use IHC Kit. COPB2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14733-1-AP targets COPB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen COPB2 fusion protein Ag6498 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coatomer protein complex, subunit beta 2 (beta prime)
Synonyms p102, Coatomer subunit beta', Beta'-COP, Beta'-coat protein
Calculated Molecular Weight 102 kDa
Observed Molecular Weight102 kDa
GenBank Accession NumberBC000326
Gene Symbol COPB2
Gene ID (NCBI) 9276
RRIDAB_3085441
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35606
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COPB2 antibody 14733-1-APDownload protocol
IHC protocol for COPB2 antibody 14733-1-APDownload protocol
IF protocol for COPB2 antibody 14733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols