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COPB2 Polyclonal antibody

COPB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14733-1-AP

Print datasheet

Synonyms

p102, Coatomer subunit beta', Beta'-COP, Beta'-coat protein

验证数据展示

  • WB analysis using 14733-1-AP

    WB analysis using 14733-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14733-1-AP (COPB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 14733-1-AP

    IHC staining of human stomach using 14733-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14733-1-AP (COPB2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPB2 antibody included
  • IF Staining of PC-12 using 14733-1-AP

    IF Staining of PC-12 using 14733-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using COPB2 antibody (14733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman stomach tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy COPB2 Ready-To-Use IHC Kit. COPB2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14733-1-AP targets COPB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen COPB2 fusion protein Ag6498 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coatomer protein complex, subunit beta 2 (beta prime)
Synonyms p102, Coatomer subunit beta', Beta'-COP, Beta'-coat protein
Calculated Molecular Weight 102 kDa
Observed Molecular Weight102 kDa
GenBank Accession NumberBC000326
Gene Symbol COPB2
Gene ID (NCBI) 9276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35606
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COPB2 antibody 14733-1-APDownload protocol
IHC protocol for COPB2 antibody 14733-1-APDownload protocol
IF protocol for COPB2 antibody 14733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols