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Conjugate
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COPE Polyclonal antibody

COPE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11457-1-AP

Print datasheet

Synonyms

Epsilon-COP, Epsilon-coat protein, Coatomer subunit epsilon

验证数据展示

  • WB analysis of HEK-293 using 11457-1-AP

    WB analysis of HEK-293 using 11457-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 11457-1-AP

    IHC staining of human stomach cancer using 11457-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPE antibody included
  • IHC staining of human stomach cancer using 11457-1-AP

    IHC staining of human stomach cancer using 11457-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPE antibody included
  • IF Staining of HepG2 using 11457-1-AP

    IF Staining of HepG2 using 11457-1-AP

    Immunofluorescent analysis of HepG2 cells, using COPE antibody 11457-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHEK-293 cells, PC-3 cells, A375 cells, HeLa cells, A431 cells, MCF-7 cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue, human prostate cancer tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy COPE Ready-To-Use IHC Kit. COPE primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11457-1-AP targets COPE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen COPE fusion protein Ag2024 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coatomer protein complex, subunit epsilon
Synonyms Epsilon-COP, Epsilon-coat protein, Coatomer subunit epsilon
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC017285
Gene Symbol COPE
Gene ID (NCBI) 11316
RRIDAB_11182386
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14579
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COPE antibody 11457-1-APDownload protocol
IHC protocol for COPE antibody 11457-1-APDownload protocol
IF protocol for COPE antibody 11457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols