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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 66994-1-Ig

    WB analysis using 66994-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66994-1-Ig (COPE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 66994-1-Ig

    IF Staining of HEK-293 using 66994-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 66994-1-Ig (COPE antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

COPE Monoclonal antibody

COPE Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66994-1-Ig

产品说明书

CloneNo. 2B8E8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP, FLJ13241

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells, LNCaP cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy COPE Ready-To-Use IHC Kit. COPE primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66994-1-Ig targets COPE in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 COPE fusion protein Ag28602 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coatomer protein complex, subunit epsilon
别名 Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP, FLJ13241
计算分子量 34 kDa
观测分子量 32 kDa
GenBank蛋白编号BC017285
基因名称 COPE
Gene ID (NCBI) 11316
RRIDAB_2882311
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO14579
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COPE antibody 66994-1-IgDownload protocol
IF protocol for COPE antibody 66994-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols