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COPE Monoclonal antibody

COPE Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B8E8

Cat no : 66994-1-Ig

Print datasheet

Synonyms

Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP, FLJ13241

验证数据展示

  • WB analysis using 66994-1-Ig

    WB analysis using 66994-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66994-1-Ig (COPE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 66994-1-Ig

    IF Staining of HEK-293 using 66994-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 66994-1-Ig (COPE antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells, LNCaP cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy COPE Ready-To-Use IHC Kit. COPE primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66994-1-Ig targets COPE in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen COPE fusion protein Ag28602 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coatomer protein complex, subunit epsilon
Synonyms Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP, FLJ13241
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC017285
Gene Symbol COPE
Gene ID (NCBI) 11316
RRIDAB_2882311
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14579
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COPE antibody 66994-1-IgDownload protocol
IF protocol for COPE antibody 66994-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols