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COPG2 Polyclonal antibody

COPG2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16111-1-AP

Print datasheet

Synonyms

2 COP, Coatomer subunit gamma 2, COPG2, Gamma 2 coat protein, Gamma 2 COP

验证数据展示

  • WB analysis of mouse heart using 16111-1-AP

    WB analysis of mouse heart using 16111-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16111-1-AP (COPG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 16111-1-AP

    IHC staining of mouse heart using 16111-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16111-1-AP (COPG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 16111-1-AP

    IHC staining of mouse heart using 16111-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16111-1-AP (COPG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells, human heart tissue, human skeletal muscle tissue, mouse brain tissue, mouse kidney tissue
Positive IHC detected inmouse heart tissue, human cerebellum tissue, human heart tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16111-1-AP targets COPG2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen COPG2 fusion protein Ag9034 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coatomer protein complex, subunit gamma 2
Synonyms 2 COP, Coatomer subunit gamma 2, COPG2, Gamma 2 coat protein, Gamma 2 COP
Calculated Molecular Weight 871 aa, 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC017443
Gene Symbol COPG2
Gene ID (NCBI) 26958
RRIDAB_10897182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COPG2 antibody 16111-1-APDownload protocol
IHC protocol for COPG2 antibody 16111-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Identification and characterization of an endoplasmic reticulum localization motif.

Authors - Yong-Wei Nie
View Article