验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, PC-3 cells |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
10464-1-AP targets COPS4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | COPS4 fusion protein Ag0735 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis) |
| 别名 | COPS4, CSN4, SGN4, Signalosome subunit 4 |
| 计算分子量 | 40; 46 kDa |
| 观测分子量 | 40 kDa, 46 kDa |
| GenBank蛋白编号 | BC004302 |
| 基因名称 | COPS4 |
| Gene ID (NCBI) | 51138 |
| RRID | AB_2081630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BT78 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an ess ential regulator of the ubiquitin conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. CSN4 gene encodes a component of CSN complex, COP9 signalosome complex subunit 4 (COPS4). The ubiquitin-proteasome system plays a major role in the rhythmic accumulation and turnover of molecular clock components. A recent study in Drosophila indicates that CSN lie in a common pathway leading to light-dependent degradation of clock protein Timeless (TIM).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COPS4 antibody 10464-1-AP | Download protocol |
| IHC protocol for COPS4 antibody 10464-1-AP | Download protocol |
| IP protocol for COPS4 antibody 10464-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuropsychiatr Dis Treat COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein. | ||
bioRxiv Crosslinking-mediated Interactome Analysis Identified PHD2-HIF1α Interaction Hotspots and the Role of PHD2 in Regulating Protein Neddylation |
