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  • Featured Product
  • KD/KO Validated

COPS6 Polyclonal antibody

COPS6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, monkey

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10256-1-AP

Print datasheet

Synonyms

CSN6, Signalosome subunit 6, SGN6, MOV34 34KD, JAB1-containing signalosome subunit 6

验证数据展示

  • IHC staining of human colon using 10256-1-AP

    IHC staining of human colon using 10256-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 10256-1-AP

    WB analysis using 10256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of 3T3-L1 using 10256-1-AP

    WB analysis of 3T3-L1 using 10256-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of COLO 320 using 10256-1-AP

    IF Staining of COLO 320 using 10256-1-AP

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • IF Staining of HeLa using 10256-1-AP

    IF Staining of HeLa using 10256-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inmouse colon tissue, 3T3-L1 cells, pig lymph node tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, COLO 320 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10256-1-AP targets COPS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, pig, monkey
Cited Reactivityhuman
Immunogen COPS6 fusion protein Ag0331 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis)
Synonyms CSN6, Signalosome subunit 6, SGN6, MOV34 34KD, JAB1-containing signalosome subunit 6
Calculated Molecular Weight 327 aa, 36 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC002520
Gene Symbol COPS6
Gene ID (NCBI) 10980
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L5N1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome.

实验方案

Product Specific Protocols
WB protocol for COPS6 antibody 10256-1-APDownload protocol
IHC protocol for COPS6 antibody 10256-1-APDownload protocol
IF protocol for COPS6 antibody 10256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Sci

CSN6 Promotes the Migration and Invasion of Cervical Cancer Cells by Inhibiting Autophagic Degradation of Cathepsin L.

Authors - Zun Mao
  • KD Validated
View Article