验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
17812-1-AP targets COQ10A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 | COQ10A fusion protein Ag12126 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coenzyme Q10 homolog A (S. cerevisiae) |
| 别名 | Coenzyme Q-binding protein COQ10 homolog A, mitochondrial |
| 计算分子量 | 247 aa, 28 kDa |
| 观测分子量 | 20-23 kDa |
| GenBank蛋白编号 | BC073923 |
| 基因名称 | COQ10A |
| Gene ID (NCBI) | 93058 |
| RRID | AB_2081926 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96MF6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COQ10A antibody 17812-1-AP | Download protocol |
| IHC protocol for COQ10A antibody 17812-1-AP | Download protocol |
| IF protocol for COQ10A antibody 17812-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol Propofol Augments Paclitaxel-Induced Cervical Cancer Cell Ferroptosis In Vitro. | ||
Mol Med Rep Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase. | ||
Mol Med Rep UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats. | ||
J Lipid Res Human COQ10A and COQ10B are distinct lipid-binding START domain proteins required for coenzyme Q function. |
