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COQ4 Polyclonal antibody

COQ4 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 16654-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

CGI-92, CGI 92

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue, Transfected HEK-293 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16654-1-AP targets COQ4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 COQ4 fusion protein Ag10089 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coenzyme Q4 homolog (S. cerevisiae)
别名 CGI-92, CGI 92
计算分子量 265 aa, 30 kDa
观测分子量 32 kDa
GenBank蛋白编号BC011895
基因名称 COQ4
Gene ID (NCBI) 51117
RRIDAB_2878296
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3A0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human coenzyme Q4 (COQ4), as a component of the coenzyme Q biosynthetic pathway, is required for the oxidative decarboxylation of the C1 carbon of Coenzyme Q in eukaryotic cells (PMID: 38295803). COQ4 deficiency manifests as an early-onset neurodegenerative disorder. COQ4 encodes a ubiquitously expressed 265-amino-acid protein that is peripherally associated with the mitochondrial inner membrane on the matrix side (PMID: 25658047, 34656997)

实验方案

Product Specific Protocols
WB protocol for COQ4 antibody 16654-1-APDownload protocol
IHC protocol for COQ4 antibody 16654-1-APDownload protocol
IP protocol for COQ4 antibody 16654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EMBO Mol Med

β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.

Authors - Agustín Hidalgo-Gutiérrez
mouseWB

Redox Biol

The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.

Authors - Pilar González-García
humanWB

Ann Clin Transl Neurol

4-Hydroxybenzoic acid restores CoQ10 biosynthesis in human COQ2 deficiency.

Authors - Diran Herebian
humanWB

J Med Genet

Human COQ4 deficiency: delineating the clinical, metabolic and neuroimaging phenotypes.

Authors - Lucia Laugwitz
humanWB

Biochim Biophys Acta Bioenerg

Characterization of human mitochondrial PDSS and COQ proteins and their roles in maintaining coenzyme Q10 levels and each other's stability.

Authors - Hsiu-Chuan Yen
humanWB

Mol Genet Metab

Detection of 6-demethoxyubiquinone in CoQ10 deficiency disorders: Insights into enzyme interactions and identification of potential therapeutics.

Authors - Diran Herebian
  • KD Validated
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