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验证数据展示

  • WB analysis using 68846-1-Ig

    WB analysis using 68846-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68846-1-Ig (CORO1C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68846-1-Ig

    WB analysis using 68846-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68846-1-Ig (CORO1C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 68846-1-Ig

    IF Staining of SH-SY5Y using 68846-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CORO1C antibody (68846-1-Ig, Clone: 1B10G4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

CORO1C Monoclonal antibody

CORO1C Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68846-1-Ig

产品说明书

CloneNo. 1B10G4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

CRN2, Coronin-3, Coronin-1C, Coronin 3, Coronin 1C

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhTERT-RPE1 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MDA-MB-231 cells, A549 cells, A431 cells, HepG2 cells
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68846-1-Ig targets CORO1C in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CORO1C fusion protein Ag6546 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 coronin, actin binding protein, 1C
别名 CRN2, Coronin-3, Coronin-1C, Coronin 3, Coronin 1C
计算分子量 53 kDa
观测分子量 57 kDa
GenBank蛋白编号BC002342
基因名称 CORO1C
Gene ID (NCBI) 23603
RRIDAB_3670439
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULV4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CORO1C also known as HCRNN4, belongs to the WD repeat coronin family. CORO1C contains 5 N-terminal trp-asp (WD) repeats and a C-terminal coiled-coil motif. It was reported to regulate actin‐dependent processes by assembling F-actin. CORO1C promoted the invasion of breast cancer and glioma cells by specifically promoting the formation of invadopodia(PMID: 30974047).

实验方案

Product Specific Protocols
WB protocol for CORO1C antibody 68846-1-IgDownload protocol
IF protocol for CORO1C antibody 68846-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols