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CORO1C Monoclonal antibody

CORO1C Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10G4

Cat no : 68846-1-Ig

Print datasheet

Synonyms

CRN2, Coronin-3, Coronin-1C, Coronin 3, Coronin 1C

验证数据展示

  • WB analysis using 68846-1-Ig

    WB analysis using 68846-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68846-1-Ig (CORO1C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68846-1-Ig

    WB analysis using 68846-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68846-1-Ig (CORO1C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 68846-1-Ig

    IF Staining of SH-SY5Y using 68846-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CORO1C antibody (68846-1-Ig, Clone: 1B10G4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

经过测试的应用

Positive WB detected inhTERT-RPE1 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MDA-MB-231 cells, A549 cells, A431 cells, HepG2 cells
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68846-1-Ig targets CORO1C in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CORO1C fusion protein Ag6546 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name coronin, actin binding protein, 1C
Synonyms CRN2, Coronin-3, Coronin-1C, Coronin 3, Coronin 1C
Calculated Molecular Weight 53 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC002342
Gene Symbol CORO1C
Gene ID (NCBI) 23603
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9ULV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CORO1C also known as HCRNN4, belongs to the WD repeat coronin family. CORO1C contains 5 N-terminal trp-asp (WD) repeats and a C-terminal coiled-coil motif. It was reported to regulate actin‐dependent processes by assembling F-actin. CORO1C promoted the invasion of breast cancer and glioma cells by specifically promoting the formation of invadopodia(PMID: 30974047).

实验方案

Product Specific Protocols
WB protocol for CORO1C antibody 68846-1-IgDownload protocol
IF protocol for CORO1C antibody 68846-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols